FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700540
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2088105.642689985785858No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1112753.0069935739108344No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAC560121.5136169315829582No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT505301.3654763899322802No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAA443751.199149313343458No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA407971.1024607219486886No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA361610.9771817086154994No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC339760.9181362720035455No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA335270.9060029076837435No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT323980.875493846843974No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC271490.7336496835596967No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTG221790.5993449604652294No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA198020.5351110918946964No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA186610.504277754057516No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA183830.49676533695082337No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT158010.42699173634118265No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC153200.4139936333616175No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC149170.4031033308652251No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC144240.3897809508882487No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA143310.387267804158312No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC136250.3681895074772871No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC135420.3659465915785264No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT129750.3506245034508477No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA126810.3426797170142736No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA126120.3408151242791593No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC126120.3408151242791593No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGACTG105310.2845800883114356No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA104800.2832019110724381No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA101290.2737168088981608No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA99700.26942013868246256No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT94410.2551249277132527No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC93110.25161192690796474No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA91980.2485583185156761No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC90690.24507234079350582No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA90220.24380225588697865No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTT89360.24147827073886513No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGACAC86720.234344176795819No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC84660.22877742167359358No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA84260.2276964983488896No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA79940.21602252644208683No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA78820.21299594113291573No Hit
CCACTACGCCTTGGCCGTACAGCAGCCTCTTACAC78670.21259059488615176No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC78650.21253654871991656No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA77150.20848308625227668No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT72740.19656590659741552No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN71560.19337718278953883No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA69070.18664843509325665No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA68970.18637820426208065No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA66810.18054121830867925No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA66210.17891983332162334No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA66160.17878471790603534No Hit
CACCCCATAAACACCACGCCTTGGCCGTACAGCAG65400.1767309635890978No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC62690.16940770806422847No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC62500.1688942694849941No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC62220.1681376231577013No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA59030.15951725964318722No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT55540.15008620363514513No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC55110.14892421106108839No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC52500.14187118636739504No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51000.13781772389975516No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT50530.136547638993228No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA49740.1344128154269377No Hit
CCGGGCGGAAACACCACGCCTTGGCCGTACAGCAG48840.13198073794635376No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG47650.1287649910553595No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA44830.12114448161619656No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA44320.119766304377199No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT43410.11730720381349748No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC43160.1166316267355575No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA42630.11519940333032477No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA41910.11325374134585763No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC40550.10957860204186416No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGACTT40110.10838958638468979No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC39760.10744377847557383No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG39600.10701140914569227No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA39450.10660606289892827No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC37810.10217427726764203No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37500.10133656169099645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)