FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002406213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7373178
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT2471903.352557065623534No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA2192402.9734803635555793No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2162102.9323854652634185No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA874851.1865304214817547No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT770831.0454515000180382No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT737601.0003827386236979No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC671800.9111403522334603No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA634900.8610940899568679No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT493330.6690873324908201No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA486130.659322208144168No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC449870.6101439569206115No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC419770.569320312082524No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA390280.5293239902793613No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA369890.5016697006365505No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN298600.40498140693199053No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC280980.3810839776281001No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC270610.3670194860343803No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA261830.3551114594005461No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT258130.35009327050018324No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGATG240070.32559908359733075No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA239830.32527357945244234No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT233370.3165120928858628No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA230560.3127009818561277No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA222910.3023255372378098No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCATG218370.29616808383033744No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA215540.292329847455195No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA214180.29048532396749405No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCTAC209200.28373111296105963No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC202290.27435930612281434No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAACAT200820.27236559323537285No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT198830.2696666213673398No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC197620.2680255379701941No Hit
TAGCTTATCAGACTGATGTTGACACGCAGGTTAAT179840.2439111059030448No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA179110.2429210307956759No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA175410.237902841895313No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA158220.21458860751768097No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC152880.20734614029391396No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC149510.20277551959277262No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT143820.19505835882437667No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA143170.19417678509863726No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCCGT142650.19347152611804572No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT136060.18453372480631824No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT133840.1815228114661005No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA133200.18065480041306478No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA132070.17912221839754852No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCATG121450.1647186599862366No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC121110.16425752911431135No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA119770.16244013097201776No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC119250.16173487199142622No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA116770.1583713291609127No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG114560.15537397849339865No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA112880.1530954494791798No Hit
TAGCTTATCAGACTGATGTTGACGCAGTGGCCGTA111630.15140011539121936No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG110450.14979972001218472No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC109350.14830782601477951No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT108500.1471549988349664No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA106940.14503922189319177No Hit
TATTGCACTTGTCCCGGCCTGTCGCACCAATTACG104060.14113317215453092No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC102740.13934289935764468No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA101840.13812225881431317No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC98870.13409414502131917No Hit
TAGCTTATCAGACTGATGTTCTGCGGACAATTACG95300.12925227086610414No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA92520.12548184785448013No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA92510.12546828518177644No Hit
CGCCTTGGCCGTACAGCAGGGCATAACCCAGACCA91520.12412558058411177No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC86790.11771043639526946No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG85630.11613716636164216No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC81390.11038659313528033No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG80650.10938295535520776No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG79480.10779612264887678No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78430.10637204201499001No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT78070.1058837857976574No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA77400.1049750867265106No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGTG76490.1037408835104754No Hit
CCGGGCGGAAACACCACGCCTTGGCCGTACAGCAG75930.10298137383906913No Hit
TAGCTTATCAGACTGATGTTGACCGCTGGTTAATG74720.10134029044192343No Hit
CCCAGTGTTCAGACTACCTGTTTCGCCTTGGCCGT74630.10121822638759026No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA74570.10113685035136816No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA74120.1005265300797024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)