FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002406214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13560133
Sequences flagged as poor quality0
Sequence length35
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2590791.910593354799691No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1781411.3137113035690726No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT1561161.1512866429849915No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1054620.7777357345978834No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT1008610.7438053889294449No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA895770.66059086588605No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA716440.5283429004715514No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN708940.5228119812689153No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA693520.5114404113882953No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN485450.35799796358929514No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC415880.3066931570656423No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT415200.3061916870579367No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA382870.2823497380151065No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA361910.26689266248347265No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAAGT305380.2252042808134699No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA261970.19319132046861193No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG261050.19251286104642187No Hit
CGCCTTGGCCGTACAGCAGTAGTACATTCAGAGAC260340.19198926736190566No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA237540.1751752729858918No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTGGCAT221310.1632063638313872No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC213530.15746895697851931No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA211420.15591292504284435No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC197040.14530830929165667No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA196760.1451018216414249No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA180230.13291167571881485No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA179940.13269781350964627No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA178580.1316948734942349No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA177750.1310827851024765No Hit
TAGCACCATCTGAAATCGGTTACGTTCCAATTACG158690.11702687576884387No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG151940.11204904848647133No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA150230.11078799890827029No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT148790.10972606242136415No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC145810.1075284438581834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)