FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151690
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT2081336.60385380541868No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1157173.6715857206768434No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA962573.0541392078535643No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA680272.1584292871443576No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT506671.6076136929710727No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA390051.2375899914014385No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT373021.1835554892771816No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA372581.1821594128864197No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA338651.0745028857533578No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN325131.031605265746314No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC301720.9573276559560109No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT269530.8551919763682341No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN249970.7931300349970968No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN232360.7372552503577445No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA207500.6583769342797039No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA207370.6579644571642516No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA204690.6494610827841572No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA171500.5441525023082854No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA155710.494052397285266No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC154700.49084776738829006No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN151700.4813290647240052No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA150780.4784099959069579No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT150630.47793406077374356No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA133270.4228525013564151No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC125930.3995634088377982No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG117440.37262548029787196No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA92900.29476249250402164No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA85020.2697600335058334No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA82420.2615104911967865No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA82260.2610028270546913No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA81400.25827413229092966No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT80670.2559579146426203No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT79840.25332440690550145No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG78910.2503736090795732No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC75640.23999822317550268No Hit
GTCCAGTTTTCCCAAAGGCTTTCTATTCCAATTAC73930.2345725626568603No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA71370.22644993638333719No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT66470.21090272203167193No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA61380.19475265651126855No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA61040.19367387020931628No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC58430.18539259889138843No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT56150.17815838486653193No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC55810.17707959856457964No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC54760.17374805263207993No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC52320.16600617446512822No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACC50590.16051705592872395No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA49050.15563078856105772No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG48660.15439335721470068No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA48320.15331457091274842No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG48270.15315592586834365No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC48160.1528069067706532No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC47090.1494119028203916No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT46400.14722260120760608No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA45750.14516021563034434No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA45700.14500157058593963No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA44640.14163829564455896No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA42870.13602226107263088No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC42400.13453099765522625No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA42220.13395987549536917No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA42100.13357912738879776No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC41430.13145328379377413No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG41130.13050141352734565No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA41010.13012066542077425No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC39900.12659874543498886No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA38310.12155383302291786No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC38230.12130000095187028No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA37970.12047504672096558No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT37960.12044331771208462No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC37480.11892032528579904No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT36330.11527148926448985No Hit
TCCCTGGTGGTCTAGTGGTTAGGATGATAAGGTTA35780.11352639377603761No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC35370.11222550441191868No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT35310.11203513035863298No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA35060.11124190513660925No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT34880.11067078297675216No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC34670.11000447379025222No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC34170.10841802334620473No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG33820.1073075080353715No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT33480.10622872173341921No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA33470.10619699272453825No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACAGACAA33050.10486437435153838No Hit
CGCCTTGGCCGTACAGCAGGGAACCGCACCTCACC32630.10353175597853849No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA32440.10292890480980046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)