FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5563614
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT2313954.159077175375574No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1986253.5700715398300455No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC858131.5423967227057809No Hit
GTCCAGTTTTCCCAGGAATCCCCCGCCTTGGCCGT767881.3801820183787012No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA617191.109332890455736No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN573891.0315057802356526No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA476370.8562240299201203No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN465450.8365965000447552No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA465320.8363628389748103No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC459380.8256863254711776No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA411880.7403101652990305No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA364560.6552575358391146No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN352370.6333473170496732No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC294720.5297276194933724No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN276370.49674546077423776No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA273190.4910297515248182No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG257660.46311624063064044No Hit
TCCCTGAGACCCTTTAACCTGCCGCCTTGGCCGTA236800.4256226258687249No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA231070.41532356486269534No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA203500.3657694441059355No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA201010.3612939359200692No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC191020.3433379813912324No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA179310.322290511167741No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT149510.26872820436500444No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA146940.26410890475147986No Hit
CAACGGAATCCCAAAAGCAGCCCGCCTTGGCCGTA144050.2589144394273219No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC138990.24981963162793108No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT133420.2398081534772182No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT121280.21798780433006315No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGCG119230.21430314899631786No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG114310.20545997619532916No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA99450.17875071850779006No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT97640.17549743745701984No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT97440.17513795888787395No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA97130.17458076710569784No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG93810.1686134228578762No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGCT93350.16778662214884066No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC92420.16611504680231232No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG90750.16311340074994418No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT89520.160902607549697No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA86300.1551150025864483No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC83820.15065746832903937No Hit
CGCCTTGGCCGTACAGCAGGCGGTGAGCCAGATTG82440.14817706620193277No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA79850.14352181873149358No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA75630.13593682092251547No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGTATTAC74560.134013610577585No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG73780.13261164415791607No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG70830.12730933526301427No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC70720.12711162204998405No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA69460.12484690706436499No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG66720.11992205066706642No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACC66280.11913119781494547No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG64710.11630929104715028No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA64250.11548249033811475No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC63710.11451189820142088No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC62630.11257071392803311No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC62540.11240894857191747No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC60870.10940730251954933No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAT60460.10867037145280029No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG60310.10840076252594089No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC59430.10681905682169898No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC57190.1027928968472651No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC57180.10277492291880781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)