FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3858318
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1582654.10191694930278No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1009662.6168397731861397No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT998892.588926055343287No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN687501.7818645326797844No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT578511.4993839284372101No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA571981.4824594551304482No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT494211.2808949391937108No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN430331.1153305663244968No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA396741.0282719050114584No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA370220.9595372906017595No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA359290.9312088842858468No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA336940.8732820882052749No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA331290.858638401500343No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN314520.8151738659177393No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA299620.7765560018640247No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT265210.6873720621265537No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN262340.6799335876410394No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA234080.6066892360868129No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC210280.5450043257191346No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC185220.4800537436261086No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA173080.4485892557326794No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA162700.4216863410429104No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA153820.39867113078807914No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC148540.3849864111770984No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA145180.37627795324283797No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA139380.36124549609441214No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT124920.3237680253416126No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT118000.3058327488817666No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA117750.30518479814261035No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC108740.2818326535034178No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT102890.26667060620716077No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT101140.2621349510330667No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGCG100340.26006150866776667No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA99150.2569772631493827No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC96370.24977205092996482No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG95110.24650637920461713No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC94000.24362947792276324No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA93750.24298152718360694No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT83750.21706349761735555No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC83170.21556025190251296No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA81790.21198356382237027No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA79410.20581507278560246No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGAGAT78870.20441549918902485No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA78640.20381938450900108No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC77740.20148676184803846No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC77510.20089064716801466No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT77180.20003535219232835No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT77160.19998351613319587No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA76170.19741763120613695No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA75010.1944111397764518No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA72700.18842407494664773No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT67860.17587974863658207No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA67010.17367671612345068No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA66610.17263999494080062No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA60540.15690775099408602No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA59860.15514532498358094No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT56330.14599626054669418No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT53720.13923165482990255No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA52340.13565496674975988No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC51040.1322856229061472No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA50320.1304195247773771No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC48820.12653182034243937No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG48180.12487306645019929No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA47960.12430286979974174No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC47840.12399185344494673No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC47350.12272186999620041No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGACGC47110.12209983728661039No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA46700.12103719807439407No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG46260.11989680477347903No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA45990.11919701797519022No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG45540.11803070664470891No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA43620.11305444496798865No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA42920.11124018289835104No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA42540.11025529777483349No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC41690.10805226526170211No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT41400.10730064240428083No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA39900.10341293796934312No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG39680.10284274131888559No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC38740.10040644653965797No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC38620.10009543018486293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)