FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837629
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1542795.436898199165571No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1086273.828090282415354No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA601042.118106348645295No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT563721.9865880987260844No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT538461.897570119279159No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA511671.8031603144738089No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT397611.4012050201065749No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN369911.3035883126370642No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA357281.2590793229136015No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT313391.104407940572922No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA294721.0386135749246994No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN265080.9341601738634613No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA252950.8914132185708561No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC232830.8205089530731466No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA166180.5856297634398295No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT165200.5821761759553487No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA155460.5478517452422427No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA154070.5429532895244585No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA153960.5425656419496699No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA146200.51521886758276No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT139410.491290440011714No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139000.48984557177841076No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA124640.43923994292418067No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA123420.4349405789128882No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA112220.3954710076616781No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA105900.37319889245563814No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT105600.3721416717971236No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA100940.3557195109015308No Hit
CGCCTTGGCCGTACAGCAGGGTATAACAGAGAGAG98540.34726174563341433No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87040.30673495372368975No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT84460.2976428560604646No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT81680.2878459446248963No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT81250.2863305950143588No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC76620.2700141561846175No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG74230.2615916316051182No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA73390.25863141376127746No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT71600.2523233304988073No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA70090.24700198651761732No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG65970.23248282280735078No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT61880.21806938116293567No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC55300.19488100805284975No Hit
CGCCTTGGCCGTACAGCAGGGTATAACAGAGTCTC53920.1900177930236828No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCTACG50780.17895221679789713No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA48920.17239744871510687No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA48480.17084685841595218No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC47870.16869717641030593No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC46930.16538455168029365No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44030.15516475198131963No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA43380.15287410722120476No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT42700.15047774039523842No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA42390.1493852790481067No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC41000.14448682333032262No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA40350.14219617857020775No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC40200.14166756824095045No Hit
TCTCCCAACCCTTGTACCAGTGCGCAGGTTAATGC39230.13824922144508672No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC39040.13757964836136086No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA38970.1373329635410408No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC37090.13070771408101622No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC36850.12986193755420458No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA34850.1228137998307742No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA34830.12274331845353992No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC34710.12232043019013408No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT34400.12122796884300238No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA34380.12115748746576807No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG34030.11992406336416776No Hit
TCTCACACAGAAATCGCACCCGTCCGCCTTGGCCG33010.11632951312521828No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA32370.11407410905372056No Hit
TACCCTGTAGATCCGAATTTCAGCGAGGTTCCGTA32370.11407410905372056No Hit
TACCCTGTAGAACCGAATTTCAGCGAGGTTCCGTA32340.11396838698786911No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA32120.11319309183829177No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA31160.10980998573104517No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC29460.10381906866612936No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT29220.10297329213931772No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA29200.10290281076208341No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA29190.10286757007346628No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT28670.10103505426537437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)