FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3657074
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2023375.532756515181263No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1068802.922555026231353No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1010522.7631926507366273No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA504021.3782056365280004No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA458341.253297034733232No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN418501.1443574836057462No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA414221.1326541382536968No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT412611.1282517116142576No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA400691.0956573479235039No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT386471.0567738033192655No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA345440.9445802846756723No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN331870.9074741172861145No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA323510.8846143118788409No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC280660.7674441370341426No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT242330.6626335698976833No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC216760.5927142846986416No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA187590.5129510641567548No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN176200.48180594650258646No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174090.47603630662108565No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA165340.45211007488500365No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT164510.44984050090318106No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA154460.42235951473773836No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA144670.39558947945816786No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA140660.3846244292568321No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA131020.35826455794988016No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA126920.3470534093649732No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA120130.3284866535377737No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC120120.3284593092729324No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC118500.3240295383686521No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA114270.3124629143408091No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT109940.3006228476645537No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA108460.29657589646805066No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGGCGT103600.28328658375520976No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGAGCA97710.2671808117637215No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA90010.2461257278359694No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA83740.22898087378051415No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGGATG79110.21632047915902167No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA74870.20472651086633742No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT73860.2019647401173725No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA72690.19876546113094784No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGATAC71750.19619510023587164No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA71060.1943083459618263No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC70940.19398021478373148No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT68460.18719883710310484No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA68060.18610506650945538No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC67510.18460113194318736No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA67260.18391752532215647No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC67200.18375345973310905No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG65840.18003463971470088No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT64280.175768934399468No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT64020.17505798351359583No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT63300.17308919644502682No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA59500.16269837580535695No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC59390.16239758889210335No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT59280.16209680197884976No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA58510.15999129358607456No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC58180.15908893284631373No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA57970.15851470328464778No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC57300.15668263754028494No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT57300.15668263754028494No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG53350.14588165292799654No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA53150.1453347676311718No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA53040.1450339807179182No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA49790.13614709464451635No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG49540.13546348802348543No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC49230.1346158158134071No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA48690.13313922551198035No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC48120.13158060241602987No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC47020.12857273328349386No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC45540.12452578208699086No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT44710.12225620810516823No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC44410.12143588015993115No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA43010.11760768308215802No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT42750.11689673219628588No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG42590.1164592239588261No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA41580.11369745320986122No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA41160.1125489940865293No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG40420.11052551848827778No Hit
TACCACAGGGTAGAACCACGGACGCCTTGGCCGTA39420.10779109200415415No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG39270.1073809280315356No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT39140.10702545258859952No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT37980.10385351786701609No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG37750.10322459977566766No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT37710.10311522271630272No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC37440.10237692756558932No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA37230.10180269800392336No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA36620.10013469784860793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)