FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6042485
Sequences flagged as poor quality0
Sequence length35
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT852231.4103965504258595No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN566510.9375447353199885No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA547850.9066634009021123No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN528300.8743091625382603No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC494090.8176933827721542No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT454120.7515451010635524No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN439090.7266712288073532No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT347630.5753096615051588No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT341800.56566131318489No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA325490.5386691071636918No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA319560.5288552640180323No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN311390.5153343367836246No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA271100.44865647163377315No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA234840.3886480479471608No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAAAG232090.38409694024892077No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC196610.3253793762003547No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC194100.3212254560830519No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG190240.3148373558229768No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA189840.3141753765214146No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA189450.3135299467023915No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA155550.257427200894996No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA150820.24959929565402314No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGATG140100.2318582503721565No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA138160.22864765075957988No Hit
AGGTGTAGAATAAGTGGGAGGCCCCGCCTTGCGGC137900.22821736421356442No Hit
AGGTGTAGAATAAGTGGGAGGCCCCGCCTTGGCCG130960.2167320233314605No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT128980.21345522578872766No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA125650.20794424810322243No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAAAC116070.19208984383080802No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC115350.19089828108799609No Hit
GGACTGTCCCCAGTGCGCCCGCCTTGGCCGTACAG112610.1863637228722951No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAGGA111390.1843446860025304No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA96990.16051343114629163No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC90120.14914393664196104No Hit
GCTCCCCGTCCTCCCCCCTCGCCTTGGCCGTACAG89970.1488956944038752No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA86680.14345091464852625No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC84940.1405713046867307No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT84920.1405382057216526No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA84250.13942939039153593No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA82820.1370628143884511No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC74280.12292955630009837No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG73470.12158904821443495No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT72090.1193052196240454No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC70110.11602842208131257No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG69930.11573053139560958No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA67870.11232133799256432No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG67310.11139456697037725No Hit
GCCGCCGGTGAAATACCACTACTCCGCCTTGGCCG66530.11010370733233099No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC64790.10722409737053548No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGATTC63970.10586703980233299No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA63120.10446033378651333No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG62210.10295433087545934No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC61690.10209375778342852No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA60660.10038916108190588No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)