FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327792
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC649362.789596321320805No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT557752.396047413170936No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA544552.3393413157189302No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA362201.555980946751256No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN343981.4777093486015933No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA340861.4643060892038464No Hit
TCTCCCAACCCTTGTACCAGTGCGCAGGTTAATGC246271.0579553499625396No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA216800.9313546914844626No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN189770.8152360692020593No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT181890.7813842473898011No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT169400.7277282506340773No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA166210.7140242770831758No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT163780.7035852000522383No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA133690.5743210733605064No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC133430.5732041350773608No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT127700.5485885336834219No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA120340.5169705884374548No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC118840.5105267137269996No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN118580.5094097754438541No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA117010.5026651865802443No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN111170.47757703437420523No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA106890.4591905118670397No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC98560.4234055276416449No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC95020.40819798332497065No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA92740.3984032937650787No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT87810.37722442555004915No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA85250.3662268793775389No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC83110.3570336181239561No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT81620.35063270257823725No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT77470.33280464921264447No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA72750.31252792345707864No Hit
CGCCTTGGCCGTACAGCAGGGTATAACAGAGTCTC67850.2914779327362582No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC64100.27536824596012016No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA61720.2651439647528645No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGGGCATG59910.2573683559355819No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA57710.24791733969358087No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC56940.24460948400888052No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA55470.23829448679263437No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT53400.22940193969220615No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA52160.22407500326489652No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT51120.21960725013231422No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC51080.21943541347336876No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA50850.21844735268443227No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA45570.19576491370362986No Hit
CGCCTTGGCCGTACAGCAGGGTATAACAGAGAGAG43900.1885907331926564No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT43650.1875167540742472No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC42610.18304900094166487No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA42590.18296308261219216No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT41750.17935451277433723No Hit
TCCCTGAGACCCTTTAACCTGTCGCAGGTTAATGC40410.1735979846996639No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA39070.16784145662499056No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC37340.16040952112559884No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC35240.15138809653096152No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA35110.15082962738938874No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA33630.14447167100840624No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG33190.14258146776000605No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC32880.14124973365317864No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC32740.14064830534686948No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA32150.13811371462742375No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT32040.1376411638153237No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG31440.13506361393114164No Hit
CGCCTTGGCCGTACAGCAGGGTATAACAGAGTCTT31150.13381779815378692No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA29380.12621402599544979No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA29120.12509708771230418No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT28610.12290617031074941No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA28360.1218321911923402No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA27860.1196842329555218No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT27250.11706372390660334No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG26830.11525943898767588No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA26600.11427137819873939No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG26030.11182270580876641No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT25290.10864372761827518No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC25220.1083430134651206No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC24950.10718311601723865No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC24690.10606617773409309No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA24600.10567954525146576No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACC24560.10550770859252029No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT24220.10404709699148379No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG24130.10366046450885646No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA24080.10344566868517463No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC23820.10232873040202904No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA23530.10108291462467436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)