FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969810
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA1010563.402776608604591No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN467021.57255851384432No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA276830.9321471743983623No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC250520.8435556483411396No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT242660.817089308743657No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT238010.8014317414245355No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT238010.8014317414245355No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT225500.7593078345079315No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC217610.7327404783470997No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA216020.7273866004895937No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN214890.7235816432701081No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA179120.6031362275701139No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT171870.5787238914273977No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC163130.5492943993050061No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147980.4982810348136749No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGTCTT147530.4967657863634374No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN141040.47491253649223353No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA124390.4188483438334439No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGTGAA111720.3761856819123109No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA107870.363221889615834No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT106370.35817106144837546No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT103370.3480694051134584No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC103190.3474633057333634No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC96290.3242294961630542No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA92750.31230954168785213No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC92090.31008717729417035No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA73470.2473895636421185No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC72670.24469578861947397No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA67880.22856681067138976No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA62370.21001343520292542No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA61370.20664621642461975No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT61290.20637683892235528No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA61140.20587175610560945No Hit
AAACCGTTACCAAATGACTCAAGCGGAACCGGCAT59860.20156171606937814No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA57970.19519767257838044No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC57310.19297530818469869No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC54570.1837491287321411No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT54520.18358076779322582No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC53680.18075230401944906No Hit
AAACCGTTACCATATGACTCAAGCGGAACCGGCAT50740.17085268081123037No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA48950.16482535919806318No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC45660.15374720941743747No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA44420.14957185813233842No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG42360.14263538744902873No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA40940.13785393678383467No Hit
CGCCTTGGCCGTACAGCAGCAATCCCACAAGAGAA38800.1306480885982605No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA37430.12603499887198172No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC37080.12485647229957472No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGAGC35570.11977197194433314No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA35550.11970462756876701No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT35410.11923321693980422No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA33240.1119263521908809No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA33060.11132025281078586No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT32310.10879483872705661No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT31850.107245918089036No Hit
GCTCCCCGTCCTCCCCCCTCCGCCTTGGCCGTACA31070.10461948744195756No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA30020.1010839077247366No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)