FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791985
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT66043223.65456834474397No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA29005110.388701945031938No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC639342.289912016002951No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG515771.8473236783148907No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA374331.3407306987680807No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA326541.169562157389814No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT314121.1250776777095866No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA305731.0950273729980642No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN276370.9898692149134038No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT207530.7433062856713055No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA193350.6925180471958123No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG166890.5977467643988058No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA161490.5784056862769678No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG159170.5700961860468449No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG155530.5570588667202725No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCCAT133350.47761717917538954No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA104570.3745363961482601No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA98090.35132710240205445No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA92890.3327023605069512No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC78280.2803739991439782No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA75340.2698438566109775No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA68890.24674201329878206No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC60370.21622609003988202No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT59930.2146501503410656No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT56260.20150538058048306No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG53950.19323169716169677No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT53370.19115432210416602No Hit
GNNTGACCCCTTTGAAGGCTTTCTATTCCAATTAC51110.18305972274206345No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC50450.1806958131938388No Hit
GNNGTCAAAAGGGTCCTTAGGGAGCGGAACCGGCA49550.17747230017353247No Hit
GNNACTGGGGAAACTTCCGAAAGATAAGGTTAATG49030.1756098259840221No Hit
GNNCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT48850.17496512337996084No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC48460.1735682677378281No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG43600.15616129742817386No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT43200.15472862497470438No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA41280.14785179719805083No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC41240.1477085299527039No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG39440.14126150391209122No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT37720.13510101236217242No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37610.13470702743746832No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA36980.1324505683232539No Hit
GTCCAGTTTTCCCAGGAATCCCTTTCGCCTTGGCC36850.13198494977587633No Hit
ATCACATTGCCAGGGATTACCATCGCCTTGGCCGT36300.13001502515235575No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCTAC32650.1169418890144467No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31670.11343184150344647No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTGTTAGCA30530.10934872501105844No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC30490.10920545776571149No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA30390.10884728965234412No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT30090.107772785312242No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG28600.10243608042306818No Hit
GTCCAGTTTTCCCAGGAATCCCATCGCCTTGGCCG28560.10229281317772122No Hit
TCCCTGAGACCCTTTAACCTGCGCCTTGGCCGTAC28280.10128994246029258No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28100.10064523985623133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)