FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4132913
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1443233.492040601870884No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA884782.1408144812145817No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC687751.664080516575113No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC465911.1273162536932182No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA454301.099224687284731No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA375420.9083665685679809No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT368730.8921794385703256No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT342860.8295843633775983No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA319910.7740545228026817No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT297570.7200006387746366No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC233470.5649042213083121No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA216370.5235290459779821No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC214570.5191737643642631No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC175480.4245915653196668No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA169710.41063046814680104No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT167460.40518636612965236No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC151930.36760996420684394No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC150330.3637386027724271No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN127350.30813617417061523No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT119630.289456855249554No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA119400.2889003470433566No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT114600.2772862627401061No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAC110970.2685031114857729No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG102030.24687187947096878No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA99370.2404357410862508No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT95030.22993467319539512No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA94090.2276602483526752No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA90880.21989332947487641No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC86710.20980359373642754No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN85280.2063435644544175No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC84400.20421431566548823No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA83430.20186730279587303No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC83170.20123820656278033No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC80880.1956973205097712No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA79220.19168078302156374No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA76050.18401064817962537No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA75440.18253469163275396No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA71780.17367895235152542No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCGCCTTGG71520.17304985611843268No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAA70950.1716706836074217No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTG70320.17014633504262008No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA70190.1698317869260737No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA68720.16627497360820323No Hit
AACCGGGCGGAAACACCACGCCTTGGCCGTACAGC66750.1615083598420775No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA66750.1615083598420775No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC63190.15289458065050002No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT62980.1523864644622328No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61950.14989427553882698No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA60380.14609550213130545No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC59800.14469213361132935No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA59210.14326456908238813No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG58680.14198218060723758No Hit
ATCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGC57130.13823179921764625No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC56390.13644129455422846No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC55990.13547345419562426No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG55770.13494114199839194No Hit
ATCCCGGACGAGCCCCCACGCCTTGGCCGTACAGC55620.13457820186391536No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55410.13407008567564815No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG55030.13315063733497415No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG53490.12942445195434793No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA52350.12666610693232594No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50330.12177851312137468No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT49350.11940730424279437No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA48280.1168183312835281No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA48130.11645539114905153No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT46760.1131405379208321No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA44890.10861588424435743No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC44290.10716412370645112No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG43840.10607530330302138No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT42090.10184100173412797No Hit
TGCCCGCATCCTCCACCACGCCTTGGCCGTACAGC41940.10147806159965138No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)