FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700725
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1252994.639457923335401No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA593322.1968915754103064No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN442071.6368567699414045No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC385871.42876449842172No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT383201.4188782641698063No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN320331.1860889205676253No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA319601.183385942663544No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC303421.1234761036388379No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA243280.9007951568560294No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA228340.8454766775588037No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT228030.8443288376269336No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT208210.770941136176397No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC207440.76809004989401No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN203890.754945431319368No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN202740.7506873154430754No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA171350.6344592655675791No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA148300.5491118125688472No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA140670.5208601394070111No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA109500.4054466856121967No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA103090.3817123179886882No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAC102410.3791944755574892No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT95630.3540901054346518No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT89320.33072600875690783No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA84600.3132492201168205No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG84560.3131011117385147No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA81440.3015486582306603No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT79540.2945135102611336No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT77400.28658971202177197No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTTAATG77120.2855529533736312No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC76920.284812411482102No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT73660.27274157865017723No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC73610.272556443177295No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA69180.2561534402799248No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA67290.2491553194049746No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA66610.24663747697377555No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT66000.2443788242046117No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA65170.24130557535476585No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG63070.23352988549371004No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC60210.2229401364448435No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC55980.2072776754390025No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA55500.2055003748993326No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA54920.20335280341389814No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC54910.20331577631932168No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT54120.2003906358477816No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA50370.1865054753816105No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA49690.18398763295041146No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT48070.17798924362902555No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47670.1765081598459673No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA46600.17254626072628645No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT45900.1699543641059345No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA45400.1681030093771117No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA44640.16528895018930104No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA44290.16399300187912505No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA41650.15421784891094056No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT39950.147923242832943No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT39890.14770108026548429No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA39660.14684945709022576No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA38870.1439243166186857No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT38590.14288755797054495No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC38350.14199890770071No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA37690.1395551194586639No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT37630.13933295689120515No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT37560.13907376722916995No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA36580.1354451119606772No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG36540.1352970035823714No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG36500.1351488952040656No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA36460.13500078682575975No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCCCGTA36000.13329754047524275No Hit
CTGACCTATGAATTGACAGCTGTATTCCATAATGC35830.132668079867443No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT35450.13126105027353765No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC35210.1303724000037027No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA35100.1299651019633617No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC34710.1285210452748799No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC34180.1265586092623277No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA34120.12633644669486896No Hit
ACTGGACTTGGAGTCAGAAGGCACGCCTTGGCCGT33880.125447796425034No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT33570.12429995649316386No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC33300.12330022493959955No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA33130.12267076433179978No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA32010.11852372973923668No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG31130.11526534541650853No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC31100.11515426413277917No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA30440.11271047589073305No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT30220.11189587981005102No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG30160.11167371724259227No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC29950.11089614825648669No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC28670.10615668015070027No Hit
CCACTACGCCTTGGCCGTACAGCAGTCATCTTCTC28190.10437937961103036No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA28080.10397208157068935No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT27800.10293532292254857No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA27690.10252802488220755No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC27540.1019726184635607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)