FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968864
Sequences flagged as poor quality0
Sequence length35
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC2763359.307768897463811No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1010653.4041640169438545No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT772162.6008601269711242No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA659252.2205463099690657No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN524851.7678479041141664No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN427601.440281535294308No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA420361.4158951033122433No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA329201.1088416310076852No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT303151.021097631956196No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT259400.8737348696336377No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT258520.8707707729286354No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT236890.7979146232363624No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC228420.7693851924507151No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN222160.748299686344676No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA193230.6508550071677247No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA187730.6323294027614602No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA135140.45519094172046953No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA133410.4493637970617718No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT125430.42248482921413716No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA121130.40800117486014853No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT120430.4056433706629876No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA118430.39890678724252776No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA112690.37957279282580814No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN111660.37610345236427134No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA110240.37132047813574487No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA109330.3682553326794356No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC106640.35919462797891716No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA103220.34767507032993095No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC79540.26791392263168673No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA75880.2555859749722453No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT73950.24908517197150154No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC73170.24645790443752225No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT72780.24514427067053257No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG72200.24319066147859922No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC65330.22005049742931976No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA64380.21685062030460137No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA60900.20512896515300127No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA60630.2042195263912392No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC59930.2018617221940783No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA59350.19990811300214492No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA57200.19266628582515063No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT56970.19189157873179774No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG54910.18495289780872415No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA52550.17700372937258158No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA51860.17467960809252292No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC50480.17003136553240566No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA50230.1691892926048482No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT48410.16305900169222975No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC47600.16033068540694353No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA46920.1580402470439872No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC46570.1568613449454067No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA43650.1470259331515354No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA42550.14332081227028248No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA40690.1370557896892549No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC40330.1358432046735721No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC38980.13129601086476173No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC38970.13126232794765944No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC38740.13048762085430657No Hit
TCGAGGAGCTCACAGTCTAGTCGCCTTGGCCGTAC38380.12927503583862382No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT37820.12738879248089505No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA37170.1251994028692456No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA37110.12499730536663181No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC34320.11559977149509038No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGGG33530.11293882104400875No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC32690.11010945600741563No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA32610.10983999267059723No Hit
TGACCTATGAATTGACAGCCGCCTTGGCCGTACAG32360.10899791974303975No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA31680.10670748138008343No Hit
CTGACCTATGAATTGACAGCACGCCTTGGCCGTAC31250.10525911594468455No Hit
TATTGCACTTGTCCCGGCCTACTATTCCAATTACG31210.10512438427627538No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA31140.10488860385655928No Hit
ACTGGACTTGGAGTCAGAAGGCACGCCTTGGCCGT30390.10236238507388684No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG30320.10212660465417076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)