FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847816
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT516861.8149346727457112No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA264910.9302216154414471No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA230320.8087601165243822No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC198180.6959017015144237No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA180720.6345915606907188No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA151320.5313545538054425No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA142040.49876817884301516No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA129780.45571764467929105No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT128660.4517848063217567No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA122860.4314183219702396No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTA119640.4201114116923284No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA114120.4007281369301949No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA111190.39043955086985954No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA110720.38878916334482283No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACCG105460.3703188689156883No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC96910.3402958618112968No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT79540.27930175264132234No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC79530.27926663801313006No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG77280.2713658466698691No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA76790.26964522988844786No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC72250.25370318868915687No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA71700.251771884138582No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66110.23214280697910258No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA66040.2318970045817567No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT65330.22940386598010545No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC65100.2285962295316832No Hit
GCCCGGCTAGCTCAGTCGGTAGAGTGCAGACAGAG62700.2201687187655382No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA60600.21279464684516133No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT60580.21272441758877678No Hit
CGCCTTGGCCGTACAGCAGCGCGAACCAATGCACT57800.2029625509513255No Hit
CTGACCTATGAATTGACAGCCACGCCTTGTAATGC57430.20166330970821147No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG57340.20134727805448102No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA56720.19917017110656024No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACCA56400.1980465030044076No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC55670.1954831351463718No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50820.17845254047312045No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT50650.17785559179385185No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT48430.17006014433516772No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC48360.16981434193782183No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGACTG47480.166724254656902No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC46650.16380974051694353No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTT46560.16349370886321307No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA46140.1620188944791377No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC44870.1575593366987193No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC43600.1530997789183009No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC43050.151168474367726No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC41910.14716540675380713No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC41910.14716540675380713No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC41850.1469547189846535No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA41720.146498228818154No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAT41510.14576082162611628No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA40880.14354860005000322No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA39460.13856232284670078No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG38800.13624475738601088No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA37900.13308444084870652No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAATGCA37670.13227680440028428No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG37390.1312935948109007No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA37390.1312935948109007No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG36600.1285195391837113No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC33800.11868744328987547No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT32500.11412254162488027No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGATGCA32070.11261261261261261No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT31990.11233169558707444No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG31980.11229658095888219No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG31960.11222635170249763No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC30550.10727518912738744No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA30380.10667824044811884No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAGCATG29710.1043255603592367No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA29300.1028858606033536No Hit
CGCCTTGGCCGTACAGCAGTCATCATGTTAGTGAT29050.10200799489854682No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA28640.1005682951426637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)