FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658862
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1686634.609711981484954No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT750982.0524961039798715No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT541991.4813075759621435No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA515211.4081154194938208No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA504991.3801832373016527No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGCT497731.3603410022023241No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA415901.1366922283485958No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA415121.1345604179660234No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN412791.1281923177206463No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN374391.0232416527324615No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA342000.9347168600510214No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC301200.823206778501075No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT277600.7587058489770864No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA259320.7087449594983358No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT254750.6962547371286482No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA194690.5321053376705653No Hit
TAGCTTATCAGACTGATGTTCTGGCTTCCAGCCGT176520.4824450881175622No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN175630.4800126378092423No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA161030.4401095203918595No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT159110.4348619871424503No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC151690.41458245760567086No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA147250.40244753696641195No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA145390.39736398913104676No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA144650.39534150235783694No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACTAATG137070.3746246783836067No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT135970.37161827912613266No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA133700.3654141642947999No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN132810.36298171398647994No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC123650.33794660744242333No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC117090.3200175355069418No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC114300.312392213753894No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT111260.3040836194423293No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT109290.29869943168121676No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGAG108720.29714157024779836No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA107640.29418983279500566No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT105930.28951624849475055No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT97150.26551971623963955No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTCGGCA94870.2592882705059661No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA92300.25226422860441305No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA91860.25106166890142345No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA90860.24832857866735614No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT86140.2354283927625584No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA84750.23162939733720483No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA82610.2257805842363008No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA82090.2243593773145858No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC80110.2189478586511325No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC74180.20274063356311334No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT71550.1955526062475163No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA71380.19508798090772486No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA66640.18213313319824578No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC65860.1800013228156733No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG65570.17920872664779378No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA61820.16895963827004135No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC61580.16830369661386518No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG61220.16731978412960097No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC60670.16581658450086392No Hit
TAGCTTATCAGACTGATGTTCTGGCTTCCAATTAC58220.15912051342739902No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA57800.15797261552909073No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC54130.14794217437006368No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC51720.14135542690596148No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC51420.14053549983574126No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT51180.1398795581795651No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA49980.13659984989868434No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA49810.1361352245588929No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA47700.1303684041650109No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT46400.12681538686072336No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC45520.12441026745474414No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGGT45200.12353567857984259No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA44990.12296172963068845No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT44810.12246977338855633No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA44340.12118522097854469No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC44150.12066593383407191No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA43030.11760487277191652No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT41310.11290395756932074No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA40970.11197470688973785No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA40680.11118211072185832No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG40500.11069015447972622No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC40420.11047150726100083No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG40250.11000688192120939No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG40210.10989755831184668No Hit
TACCACAGGGTAGAACCACGGACGCCTTGGCCGTA40080.10954225658141793No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG39770.10869499860885706No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC39150.10700048266373535No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA38760.10593457747244908No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGCGA36990.10109700775814993No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA36830.10065971332069916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)