FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5005093
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC3613947.220525173058723No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1526883.050652605256286No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1512833.022581198790912No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA739951.4783941077618337No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT703021.4046092650026683No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA693801.3861880288737891No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA627691.2541025711210563No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN561641.1221369912607018No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA550171.099220334167617No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA547611.0941055440927872No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT546921.0927269483304307No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA530531.0599803040622822No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN495610.9902113706978072No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA439440.8779856837824991No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT436220.8715522368915023No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT396140.7914738047824487No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC381800.76282298850391No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC294740.5888801666622379No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT247790.4950757158758089No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA243820.48714379533007673No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN233520.46656475713837886No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN224330.4482034599556891No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA219350.43825359488824683No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA205730.411041313318254No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT178890.3574159361274606No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA176470.35258086113484804No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA169170.33799571756209124No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA163800.3272666461941866No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC159210.31809598742720663No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA154840.30936488093228237No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA147960.29561888260617736No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT144070.28784679925028367No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT132210.2641509358567363No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA118790.23733824726133962No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA117680.23512050625233136No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA112970.2257100917005938No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA112750.2252705394285381No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT111210.22219367352414832No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC107200.21418183438349697No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC105960.2117043579410013No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA104040.20786826538487896No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT101530.2028533735536982No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA100890.20157467603499077No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT98000.19580055755207743No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA92770.18535120126639007No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG92310.18443213742481906No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA92230.18427230023498065No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC90910.18163498660264654No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA89740.17929736770125948No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC89720.1792574084037999No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT88910.17763905685668577No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT85320.17046636296268622No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC83940.1677091714379733No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA83240.166310596026887No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA81160.16215482909108783No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT80530.1608961112211102No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC79270.1583786754811549No Hit
TCCCTGAGACCCTTTAACCTGTGCGCCTTGGCCGT77900.15564146360517178No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA72700.14525204626567378No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG69810.13947792778276047No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA69390.13863878253610873No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC68710.13728016642248206No Hit
AACCCGTAGATCCGATGAACACGCGGAACCGGCAT68180.13622124503980246No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA67670.13520228295458248No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA67600.13506242541347382No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT66750.1333641552714405No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC66320.13250503037605896No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG65960.13178576302178602No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC64990.12984773709499503No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA63190.12625140032363036No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC62840.1255521126180872No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC62480.12483284526381429No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA62340.12455313018159703No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC61880.12363406634002605No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA60920.12171602006196489No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC59530.11893884888852216No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA58550.11698084331300136No Hit
CGCGACCTCAGATCAGACGTGGCGCCTTGGCCGTA57810.1155023493069959No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT57180.11424363143701825No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA56760.11340448619036649No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT56710.11330458794671747No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC56260.1124055037538763No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA55930.11174617534579277No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA55680.1112466841275477No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA55090.11006788485248926No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA54550.10898898382107984No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG54340.10856941119775397No Hit
CGCGACCTCAGATCAGACGTGCGCCTTGGCCGTAC54220.10832965541299631No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG53620.10713087648920809No Hit
AACCCGTAGATCCGAACTTGTGACGCCTTGGCCGT52840.10557246388828338No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA52560.10501303372384889No Hit
TACCCTGTAGATCCGAATTTGTGCGCCTTGGCCGT52020.10393413269243948No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC51130.10215594395548695No Hit
AACCCGTAGATCCGAACTTGTCGCCTTGGCCGTAC51040.1019761271169187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)