FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5968240
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA1480812.4811502218409447No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1113581.86584319665429No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT983561.647990027210702No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN933921.5648164282937682No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT756821.2680790316743293No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT703801.1792421216304976No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA678761.1372867042880315No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT630731.056810718067638No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA605911.0152239186091712No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA600341.0058911840006435No Hit
TAGCTTATCAGACTGATGTTCTGGCGAGGTTAATG574200.9620926772381808No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA479200.8029167727839364No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA365600.6125759017733872No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA359380.602154068871225No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT350930.5879957910539791No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN323040.5412650965778856No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC306530.5136019999195743No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN302880.5074862941168586No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA278630.46685455008511717No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA275200.4611074621664008No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA264880.4438159323351608No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA264620.44338029301770704No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC256070.42905446161682503No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA253650.42499966489283275No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN241460.40457488304759864No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA237560.3980402932857928No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC232930.39028256236344383No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGGT229820.38507164591236276No Hit
TAGCTTATCAGACTGATGTTGACCGCTCCAATTAC218070.36538409983512726No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA217830.36498197123440074No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA196640.32947736686192247No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG180780.3029033684972454No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC176660.29600016085144026No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC169780.28447247429728023No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA163160.27338042706057397No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA157330.26361205313459246No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT152370.2553013953862445No Hit
TAGCTTATCAGACTGATGTTCTGGCGCTTGGCCGT151970.25463118105170035No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC148290.2484652091738938No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC145000.2429526962722679No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC139380.23353618487192204No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA135040.22626435934211764No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA133490.22366727879575887No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA132760.22244413763521576No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC129740.21738401940940713No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG126900.2126254976341434No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG126160.21138560111523666No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA123120.20629197217270084No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAG122020.20444888275270431No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA119540.20029355387853037No Hit
TAGCTTATCAGACTGATGTTCTGTATTCCAATTAC119350.19997520206962185No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT117750.1972943447314451No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT112950.18925177271691487No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG111800.1873249065051003No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA111610.18700655469619185No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC109170.1829182472554723No Hit
TAGCTTATCAGACTGATGTTCGTTATTCCAATTAC107170.17956717558275137No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC102690.17206077503585646No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC97210.1628788386526011No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC94760.15877377585351796No Hit
TCCCTGAGACCCTACAGGTGTCATGGAACCGGCAT93610.15684690964170342No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT91870.15393147728643622No Hit
TAGCTTATCAGACTGATGTTCTGGCGAGGTTAAAC89900.1506306716888061No Hit
CGCCTTGGCCGTACAGCAGCGTGTCGCACAGACAC88050.14753093039153922No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT87970.14739688752463037No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC86750.1453527338042706No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC82180.13769553503210327No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC81650.1368075010388322No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA78460.13146254172084232No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG77430.12973673980939104No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC77070.12913354690830126No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC75450.12641917885339732No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA75350.12625162526976125No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG75190.1259835395359436No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA74570.12494470731740011No Hit
TACCCTGTAGATCCGATAAAGTCGCTCCAAGGCAT74110.12417396083267429No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG73880.12378858759031139No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC73860.12375507687358417No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC71790.120286717692318No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC71580.11993485516668231No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA71170.1192478854737745No Hit
CCCAGTGTTCAGACTACCTGTTTCGCCTTGGCCGT71020.11899655509832044No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT70320.11782368001286812No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA68100.11410399045614789No Hit
TAGCTTATCAGACTGATGTTCTGCGCCTTGGCCGT66710.11177499564360682No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG66460.11135611168451671No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT66230.1109707384421538No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG65660.11001568301542833No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTTAATG65540.10981461871506508No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC63390.10621221666689007No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCCGT63200.10589386485798159No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA62200.10421832902162112No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG61740.1034475825368953No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC61130.1024255056767154No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA60500.10136991809980832No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT59840.10026406444781041No Hit
TAGCTTATCAGACTGATGTTCTGTATCTTGGCCGT59800.10019704301435599No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)