FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234292
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1242945.56301504011114No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT528662.366118663093275No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA477632.13772416497038No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA327881.4674894776510858No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT258791.1582640048838737No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT238411.0670494277381828No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA237331.0622156817461639No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA187550.8394157970399573No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC186780.8359695151752771No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA152430.6822295384846744No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA147190.6587769190419157No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC139460.6241798296731135No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC118800.5317120591220843No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA112230.502306771003969No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC110130.49290782046393217No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC100030.44770334405708834No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT99410.4449284158024108No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA96120.43020339328968643No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC95190.42604100090767005No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC94460.422773746672324No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA93590.4188798957343086No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT82750.3703634081847852No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC76920.3442701312093495No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA76150.34082384934466936No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC73850.33052976065796236No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA71510.32005664434192127No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT61520.275344493915746No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC57770.2585606536656802No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA54070.242000597952282No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA52680.23577938783292426No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA51100.2287077964742299No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCA50510.22606713894155286No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA50180.22459016099954707No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC49610.22203901728153705No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA48820.21850322160218988No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA48220.21581780716217933No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA46960.21017843683815723No Hit
CCCCGGCATCTCCACCACGCCTTGGCCGTACAGCA45660.20436003888480112No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT45350.20297257475746233No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT45000.20140608300078952No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA44720.20015288959545127No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG44310.19831785639477742No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA43590.19509535906676476No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAT43210.19339459658809144No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC42910.19205188936808618No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGCG41030.18363759078938652No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA39260.17571561819135548No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38250.17119517055067107No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC38230.17110565673600409No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA37690.1686887837399946No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC36920.16524250187531442No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA36720.16434736372864425No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC34780.15566452370594355No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC32200.14411724161389827No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG31600.14143182717388775No Hit
GCCCGCATCCTCCACCACGCCTTGGCCGTACAGCA30640.1371351640698709No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC30230.13530013086919704No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC29940.13400218055652527No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC29440.13176433518984984No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT29320.13122725230184776No Hit
CGCCTTGGCCGTACAGCAGTATAACCTATAGAGAC28920.1294369760085074No Hit
CCGGGCGGAAACACCACGCCTTGGCCGTACAGCAG28840.12907892074983932No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA28390.12706485991983144No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA28000.1253193405338246No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA27950.12509555599715705No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA27800.1244242023871544No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA27690.12393187640648581No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT27300.12218635702047898No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA26470.11847153371179775No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC26240.11744212484312705No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26110.11686028504779143No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT25890.11587563308645422No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC25200.11278740648044212No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG25130.11247410812910758No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA24950.11166848379710441No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG24350.10898306935709387No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT24200.10831171574709125No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC23690.10602911347308229No Hit
AACCGGGCGGAAACACCACGCCTTGGCCGTACAGC23680.10598435656574878No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC23440.10491019078974458No Hit
TGGCTCAGTTCAGCAGGAACGCCTTGGCCGTACAG23430.10486543388241107No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC23090.10334369903307178No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT22890.1024485608864016No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC22630.10128488129573036No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT22600.10115061057372984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)