FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449563
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1205203.493775878277915No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA718372.082495666842438No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA521251.5110609662731191No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA463131.342575856709966No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT393841.1417098339702738No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT338790.9821244024243071No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA238150.6903773028641599No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC224210.6499663870467071No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGATG219850.6373271049115498No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA190570.5524467881873732No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA178910.5186454052295899No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT176820.512586666774893No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC129900.37656943792590536No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC123350.3575815255439602No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC115640.33523086837376215No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT114980.33331758254596305No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA112780.3269399631199662No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA111210.3223886619841412No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC110130.31925783062956087No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA106600.30902465036875687No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA100500.2913412510512201No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA99050.287137820065904No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC96380.2793977092170805No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN96050.278441066303181No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA91750.26597571924327806No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGAAAC89230.2586704460825907No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT85460.24774152552076883No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT85250.24713275275737825No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA84310.24440776991172505No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG84090.24377000796912537No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC83430.2418567221413263No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG82430.23895780422041865No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA82190.23826206391940077No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG77730.22533288999215265No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG76930.2230137556554265No Hit
TAGCACCATCTGAAGCTAACCTATTCCAATTACGT76920.22298476647621743No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA76440.22159328587418176No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA73100.21191090001835014No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC69590.20173569811596423No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC65260.18918338351843408No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC61940.17955897602102064No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC60680.175906339440677No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC58960.1709202006167158No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC58250.16886196889287136No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC54690.15854182109444007No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA54270.15732427556765888No Hit
TGTGCAAATCCATGCAAACAGTATAAGGTTAATGC53860.1561357192200867No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT52800.1530628662239246No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC52420.1519612774139797No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC51650.14972911061488078No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA51590.14955517553962633No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT49540.14361239380176563No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT47830.13865524415701352No Hit
CGCCTTGGCCGTACAGCAGAACGTCTCCCAGATTG46850.135814304594524No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA46030.13343719189937972No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC45910.13308932174887078No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT44950.13030636054479944No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG44630.129378706810109No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT44010.12758137769914624No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC43250.1253782000792564No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43220.1252912325416292No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA41760.12105881237710399No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA41590.1205659963305497No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC41260.11960935341665017No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC40020.11601469519472467No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG40010.11598570601551558No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC38640.11201418846387208No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC37650.10914425972217351No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC36850.10682512538544738No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG35650.10334642388035817No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA35180.10198393245753158No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT35170.10195494327832251No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG34680.10053447349707774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)