FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564401
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1366633.8341084518829396No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1006492.823728306663588No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN715522.0074060129598212No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT668371.8751257223864541No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA510531.4323023700195348No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA502061.4085396115644677No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN399711.1213945905637441No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT388771.0907021965261485No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT386291.0837445057388324No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC358301.005217987538439No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN249980.7013240092795395No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA235930.6619064465530112No Hit
CCACTACGCCTTGGCCGTACAGCAGCTTACACCAC232250.6515821311911876No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA215440.6044213319432915No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA206520.5793960892727839No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205250.5758330782647632No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA198380.5565591525757063No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA197290.5535011352538618No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA195040.5471887141766597No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT191260.5365838467669604No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT190100.5333294430116028No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA175810.493238555370173No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA165120.4632475414522665No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA160700.4508471409361629No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA158650.44509582395471214No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC139760.3920995421110026No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA138430.3883681998742566No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC135450.38000774884756233No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA128450.3603691054962671No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC126690.35543138945365577No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT126590.3551508374057801No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA117840.3306025332166611No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT113890.3195207273255731No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC110900.3111322210940912No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC104020.29183024020024684No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA101210.28394672765494117No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT99200.2783076314926407No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA95330.2674502672398532No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA94500.26512168524248536No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA94260.2644483603275838No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGACAC94220.26433613950843354No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA93110.2612220117770139No Hit
GCATTGGTGGTTCAGTGACGAGGCCTCTATTCTAT85080.23869368233259952No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC83450.2341206839522265No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT79820.2239366446143405No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT79400.22275832601326281No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA77570.21762422353713848No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA77170.21650201534563593No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC75160.2108629191833354No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA75120.2107506983641852No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGAGTG75070.21061042234024732No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC74490.2089832204625686No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA73930.20741212899446498No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA73080.20502743658752198No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG68360.19178537992779152No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC66450.18642683581336666No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG65270.18311632164843406No Hit
TTCAAGTAATTCAGGATAGGTTCGCCTTGGCCGTA64980.1823027207095947No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA64880.18202216866171902No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA64640.18134884374681748No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG62700.17590613401802996No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT61920.17371782804459993No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT61560.1727078406722476No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA59280.16631125398068286No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA59280.16631125398068286No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC59280.16631125398068286No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA58950.16538543222269325No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG57630.1616821451907347No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA57500.16131742752849637No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT56340.15806302377313888No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA56100.15738969885823734No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC55830.15663220832897307No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC55370.15534166890874512No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC53150.14911341344590576No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAAAC50180.14078101762399908No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA50130.1406407416000613No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAATG49330.1383963252170561No Hit
TGAGATGAAGCACTGTAGCTCGCCTTGGCCGTACA49230.13811577316918047No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG49120.13780716591651726No Hit
GTGAAATGTTTAGGACCACTAGCGCCTTGGCCGTA48790.13688134415852762No Hit
TCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTAC47250.13256084262124268No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC45670.12812812026480747No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC44500.1248456613046624No Hit
TGTAACAGCAACTCCATGTGGACGCCTTGGGGCAT43860.12305012819825828No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT43760.12276957615038264No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA43300.12147903673015467No Hit
TAACACTGTCTGGTAACGATGTCGCCTTGGCCGTA43120.12097404304397849No Hit
CAGTGCAATGTTAAAAGGGCATCGCCTTGGCCGTA42940.12046904935780232No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCCGT41120.11536300208646558No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA40600.11390413143751223No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA38270.10736726872200968No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC37500.1052070179533672No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA37310.10467396906240348No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG36520.10245760788418587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)