FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3858375
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT2601666.742890465545728No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA1073332.7818187708556064No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC823842.135199403894126No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT803902.083519616418829No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN718901.8632196196585349No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN463331.2008423235170247No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT414051.0731201606894094No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA406411.0533190786276605No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC369560.9578125506204037No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA346680.898512975021868No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA323250.8377879288560599No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC322840.8367253053422748No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA319130.827109858424855No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN301300.7808986943985486No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG290100.7518709301195452No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA243330.6306540966080281No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229040.5936177795056209No Hit
CCACTACGCCTTGGCCGTACAGCAGCAAGCACCGC224380.5815401561538213No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA219400.5686331681083358No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA206250.5345514627271843No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC197110.5108627336637833No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG188790.4892992516279521No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA176420.4572391226876599No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC169050.4381378170862086No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG168230.4360125700586387No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA165050.4277707584151359No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA151200.3918748177665468No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA141090.36567207697541065No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA134870.3495513007418926No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA133470.3459228302070172No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC130980.3394693361842745No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG127980.33169404218097No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA127190.32964654809343313No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGATAC126830.32871351281303657No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA126340.3274435481258302No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT125470.32518871286487183No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC119930.31083033660543624No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG118320.30665759549032945No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA117440.3043768425826935No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA110770.2870897722486798No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA98600.25554799624194124No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC95960.24870573751903327No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC93090.24126737292253864No Hit
CGCCTTGGCCGTACAGCAGCAAGCACCGCAGAGCA86800.22496517316227685No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT85020.22035183205364953No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC84570.21918553795315385No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT84410.21877085560631096No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA84060.2178637379725921No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC83630.21674927916545175No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT80330.2081964557618168No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA79790.20679690284122204No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT78190.20265007937279295No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG72630.18823986782000193No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG71120.184326303171672No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG71070.1841967149382836No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT69960.18131985615706095No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC69040.17893543266271422No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTT66830.1732076327469466No Hit
CCCAGTGTTCAGACTACCTGTTCCGCCTTGGCCGT66420.17214500923316164No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC63650.1649658211034438No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGCC62210.16123367998185764No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTCGC62180.1611559270418246No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA60840.15768296238701526No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT59790.1549616094858587No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA58890.15262902128486733No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAAAC58600.15187740953121456No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC55630.14417986846794312No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC55480.1437911037677779No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACATA55290.14329866848090195No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC55130.14288398613405903No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC54730.14184728026695176No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC52320.13560112741763047No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACGCC51960.13466809213723394No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG51840.13435708037710176No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA51730.13407198626364727No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACATA51300.13295752745650694No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG50910.13194673923607736No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT49920.1293808922149869No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT49530.1283701039945573No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA48720.1262707746136651No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT48610.12598568050021058No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC48510.12572650403343377No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACGCC48230.12500080992645868No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA46970.12173518644507078No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA45070.11681083357631128No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC43400.11248258658113842No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC43020.11149771600738653No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT42490.1101240807334694No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA42460.11004632779343636No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT41800.10833576311270936No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA41660.10797291605922184No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA41230.10685845725208151No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCCGT40780.10569216315158585No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT40640.1053293160980983No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT40200.10418893964428029No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT40120.10398159847085885No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAAAC39800.10315223377717303No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC39340.10196002202999968No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)