FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3072858
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1897646.1754887469580435No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT609891.9847646718462095No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA588601.9154806372438948No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA481541.5670753415875385No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA268970.8753089143722229No Hit
GTCCAGTTTTCCCAGGAATCCCCCGCCTTGGCCGT248260.8079123734321599No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA236730.7703903011463595No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA191260.6224173066246471No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC153070.4981356118636136No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA148250.48244988867041694No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA135000.43933042138621436No Hit
TCCCTGAGACCCTTTAACCTGCCGCCTTGGCCGTA126140.41049732854560805No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC121630.39582043817189083No Hit
CAACGGAATCCCAAAAGCAGCCCGCCTTGGCCGTA111620.3632449010009574No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA108930.3544908355674099No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA108480.3530264008294558No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG105810.3443374213842618No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT103330.33626675882842616No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC103190.33581115690995156No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT93750.30509057040709336No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC78030.25393298356123195No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT74180.24140393080318065No Hit
CTGACCTATGAATTGACAGCCATAAGGTTAATGCA72510.23596925077566228No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC68190.22191067729130343No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA66410.21611802432784075No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61650.20062755909970456No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA61090.1988051514258062No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA60790.1978288616005035No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA56400.18354248715690735No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT54020.1757972545428393No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA49980.16264988489542961No Hit
CTGACCTATGAATTGACGATCGCCTTGCGGCATGT47090.15324495957834694No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA46550.15148763789280206No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC44400.1444908941447994No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC43280.14084607879700264No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC43180.1405206488552351No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC41530.13515105481607026No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT38780.1262017314174622No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA38160.12418406577850327No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT37510.12206877115701409No Hit
CTGACCTATGAATTGACGATCGCCTTGCGGCATGG37030.12050670743652978No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA36450.11861921377427789No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT36110.11751275197226815No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC35720.11624357519937467No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGG35710.11621103220519789No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA35090.11419336656623899No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC34950.11373776464776439No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA34820.11331470572346655No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA34110.11100415313691685No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC33760.10986514834073036No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33500.10901903049213468No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC33470.10892140150960443No Hit
CGCCTTGGCCGTACAGCAGGCGGTGAGCCAGATTG32270.10501624220839363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)