FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002406236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3597332
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1401703.8964988497030575No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA670501.8638813431732186No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA604891.6814961754989532No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT429051.1926894709746No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA419721.1667535829331293No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT405531.1273076824713426No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT376641.0469981641950201No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT316080.8786511781509185No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA271280.7541144381447139No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA270980.7532804867607438No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA261280.7263160586790433No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA216000.6004449964584865No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA202110.5618330473806699No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC199380.5542440897865418No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC177940.49464436421214386No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA177370.49305985658260065No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA165600.4603411639515063No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTG165080.4588956482192914No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG149990.41694789360559437No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC143350.39848976964038907No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC138410.38475737018434775No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA130660.363213626098453No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT129300.35943304649112173No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA125610.34917544446828924No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT123490.3432821880215671No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA114220.3175130902568904No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA113110.314427470136201No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC110680.30767246392604297No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC109920.305559787086652No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC107180.2979430311130582No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT107050.2975816521800045No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT105930.2944682336798494No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT105250.2925779438761838No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA104340.2900482913448078No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGTC100210.27856756062548577No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC96770.26900491808929505No Hit
CATGCCTTGAGTGTAGGACCGTCATTCCAATTACG95440.26530773362036086No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA92110.2560508732582925No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG91240.25363241424477917No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN90580.2517977212000449No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC89490.2487676978382868No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA84900.236008241663544No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC82830.23025397711415016No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGCA76790.21346375591688505No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA74200.20626397563527638No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT73940.20554121776916895No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA71770.19950896942511842No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA69720.1938103016346559No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC68430.1902243106835844No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC67260.18697190028610092No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT66850.18583216672800842No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG66480.1848036266877786No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT65280.18146782115189813No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT64750.17999450704021758No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA60680.16868056659768962No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT60450.1680412038699792No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC58940.16384364857066291No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA58650.16303749556615849No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA58490.16259272149470774No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTTG57590.16009086734279737No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC56670.15753341643195568No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA54560.15166795836469918No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC54530.15158456322630215No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC54450.1513621761905768No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA54180.1506116199450037No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA53910.14986106369943059No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA51390.14285587207408157No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC50590.1406320017168279No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA50210.13957566329713245No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50160.1394366713998041No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG49470.1375185832166728No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC49000.1362120593817863No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG46170.12834511799300147No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA45460.12637143305093887No Hit
TACCCATTGCATATCGGAGTTGTCGCCTTGGCCGT45390.12617684439467916No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC44670.12417536107315087No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA44660.12414756269368522No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA44430.12350819996597479No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCCCA43680.1214233215060495No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG43180.12003340253276594No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA42680.11864348355948241No Hit
TAGCTTATCAGACTGATGTTCTGGCGGTTGGCAAC42470.11805971759070333No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG42040.1168643872736795No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGTAC41970.1166697986174198No Hit
CATGCCTTGAGTGTAGGACGCAGTAAGGTTAATGC41920.11653080672009145No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC41650.11578025047451834No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG41360.1149740974700139No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATTTG40670.11305600928688261No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA40520.11263903359489756No Hit
TAGCTTATCAGACTGATGTTCTGCGGACAATTACG40350.11216646114398114No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT40140.11158269517520206No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC38950.10827468801878726No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG38750.10771872042947385No Hit
TATTGCACTTGTCCCGGCCCACTAAAGGTTAATGC38060.10580063224634258No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC38050.1057728338668769No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT37560.10441071327305902No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC37550.10438291489359337No Hit
TAGCTTATCAGACTGATGTTGACATAACCAATGGT37310.10371575378641726No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG37210.10343776999176056No Hit
CGCCTTGGCCGTACAGCAGAAAGCTTGACAGAGAG36900.10257602022832478No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG36880.10252042346939343No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)