FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209742
Sequences flagged as poor quality0
Sequence length35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT721473.2649512929563724No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC640752.8996597792864507No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA487922.208040576682708No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN378711.7138199844144701No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA377361.7077106739157786No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA356671.6140798337543476No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN281461.2737233577494567No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT254841.1532568055456247No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT253221.1459256329471947No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA237561.075057631162371No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA224671.0167250294378256No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA198670.8990642346482077No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA171440.775837179182004No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN161390.7303567565806325No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN155280.7027064698050722No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGGG145430.6581311302405439No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC132240.5984409039607339No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA129070.5840953378267689No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGAA120470.5451767672425106No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA98160.44421475448265No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC96850.4382864605913269No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA90290.40859973698286944No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA89890.40678957090918305No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG87620.39651687844101263No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT87460.395792812011538No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA86160.3899097722720571No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT85450.38669672749126366No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA85220.385655881998894No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT83550.37809843864125314No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA82230.3721248905980879No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC78950.35728152879385916No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA77420.3503576435620086No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA70600.319494312005655No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGGC68530.3101267025743277No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC68170.3084975531080099No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT66150.2993562144358934No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA64800.2932469039372017No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA59620.26980525328296245No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGAT57210.2588990026890017No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT53070.24016378382634715No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC52760.23876090511924017No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC51280.2320632906466004No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC48340.2187585700050051No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC47970.21708416638684516No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT46940.2124229887471026No Hit
CGCGACCTCAGATCAGACGTGGCCGCCTTGGCCGT44710.20233131288630077No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC44710.20233131288630077No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA42950.19436658216208047No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC40460.18309829835338243No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT39870.18042830339469496No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT39830.18024728678732632No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA39120.1770342420065329No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA38710.1751788217810043No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG38160.17268984342968544No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG37830.17119645641889414No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC37290.16875273221941747No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA37180.1682549365491537No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC36460.16499663761651814No Hit
CGCGACCTCAGATCAGACGTGGCGCGCCTTGGCCG36430.16486087516099165No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC36350.16449884194625436No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG35870.16232664265783064No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG35590.16105952640625015No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG35150.15906834372519507No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT34350.1554480115778222No Hit
CCACTACGCCTTGGCCGTACAGCAGTAACCCTTTT34200.1547691993001898No Hit
CGCGACCTCAGATCAGACGCGCCTTGGCCGTACAG33400.15114886715281692No Hit
CGCGACCTCAGATCAGACGTGCGCCTTGGCCGTAC32550.14730226424623327No Hit
AACCCGTAGATCCGATCTTGTGCGCCTTGGCCGTA32530.14721175594254896No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT32010.14485854004675658No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC31120.1408309205328043No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA30830.1395185501293816No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA30160.13648652195595684No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC29940.13549093061542933No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA29780.13476686418595474No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT29730.13454059342674393No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA29620.13404279775648015No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT28860.13060348221647594No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA27980.1266211168543658No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC27240.1232723096180459No Hit
CGCCTTGGCCGTACAGCAGTAACCCTTTTAGAGGT26830.12141688939251731No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC26820.12137163524067515No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC26710.12087383957041138No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA26560.12019502729277898No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC26510.11996875653356817No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG26290.11897316519304064No Hit
ACCACAGGGTAGAACCACGGACACGCCTTGGCCGT26260.11883740273751416No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG25600.11585062871593153No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT25460.1152170705901413No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA25280.1144024958569824No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA24960.11295436299803327No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA24470.11073690955776738No Hit
CGCGACCTCAGATCAGACGTGGCGCCTTGGCCGTA24180.10942453915434472No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA24100.10906250593960742No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC24040.10879098102855446No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT23630.10693556080302587No Hit
ACCCTGTAGATCCGAATTTGTGACGCCTTGGCCGT23410.10593996946249834No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT23040.1042655658443384No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT23000.10408454923696975No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC22370.10123353767091361No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG22320.10100726691170281No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)