FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290557
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN747282.2709833016112473No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC605501.840113998936958No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT347691.0566296222797538No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA330731.0050881963144842No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN315240.9580140991327608No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT293660.8924324969906311No Hit
CCACTACGCCTTGGCCGTACAGCAGTTTTCCTCTT240710.7315174908077873No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT234120.7114904862611406No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA231330.70301167856992No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA229970.6988786396953465No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN226730.6890322823765095No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA190240.5781392025726951No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA186710.5674115354938389No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA180510.5485697406244596No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA178270.5417623824781033No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN166480.5059325822345578No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA155210.4716830615607024No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT154430.46931264220616753No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT151500.46040837463079953No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT148810.45223346685682697No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC136840.4158566467622351No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAA122090.37103140896814735No Hit
TTCACAGTGGCTAAGTTCCGCGCCTTGGCCGTACA120280.3655308204659576No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAT119450.36300845115279873No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA112950.34325495653167537No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA109660.3332566492542144No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA107110.32550720136438904No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC105910.3218604023574124No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC100330.30490278697497114No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC95930.2915311906160568No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA87540.2660339875589452No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA84390.25646114016563154No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA83610.2540907208110967No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC80230.24381890360811254No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA78550.23871338499834527No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA74910.22765142801051616No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC74840.22743869806844252No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT68540.20829300328181521No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA66040.20069550535061392No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG63610.1933107373614862No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC62870.19106187797385063No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA61790.18777975886757164No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC60220.18300853016677723No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT60030.18243112032400594No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC57180.17376997268243644No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA56350.1712476033692776No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC56200.17079175349340553No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA54140.16453141519809564No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA51500.15650845738274705No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC51470.15641728740757266No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT50990.15495856780478198No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA50300.15286165837577043No Hit
TTCACAGTGGCTAAGTTCCGACGCCTTGGCCGTAC47630.14474753058524742No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC45770.13909499212443366No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT44200.13432376342363922No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGC43640.13262192388705013No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA42850.1302211145407905No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT42770.12997799460699208No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC41910.1273644553186588No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG41710.12675665548416273No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA41150.12505481594757362No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA39590.120313977238504No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA39560.1202228072633296No Hit
CCACTACGCCTCCGCCTTGGCCGTACAGCAGTTTT39140.11894642761088776No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA38460.116879908173601No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGG38280.11633288832255452No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC38040.11560352852115918No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG37990.11545157856253516No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC37930.11526923861218633No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA37250.11320271917489956No Hit
TGGAGAGAAAGGCAGTTCCTGACGCCTTGGCCGTA36170.1099206000686206No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA34050.10347792182296188No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC33670.10232310213741928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)