FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650935
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA887063.3462155805404508No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN479451.8086071518162459No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT470681.7755244847572647No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA452651.7075107462084134No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT400551.510976315903634No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA352131.3283237801002288No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN324901.225605305297942No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGCT305431.1521595210746396No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA269281.0157925411222835No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA267471.008964761489814No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT255420.9635091015056952No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC231030.8715038278946862No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT212250.8006608988903916No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA206350.7784046006409059No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205950.7768956990646696No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA200880.7577703715858745No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA183680.6928876038077131No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA167910.6333991591645967No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA167310.6311358068002422No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA162010.611142860915111No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143210.5402244868320045No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC124380.4691929451306803No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA110060.41517426870142043No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA103390.39001333491768No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT100740.3800168619751145No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA92300.34817903871652833No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA89160.3363341613430733No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC87920.33165656645674074No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT86750.32724302934624955No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACTAATG82780.31226718120210417No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT81860.30879670757676064No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC77980.29416036228726844No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA76360.2880493109035114No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA74760.2820137045985662No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC74170.2797880747736176No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT72540.2736393008504547No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC70150.2646236139324427No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA69860.2635296602896714No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC69630.26266204188333553No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGAG67030.2528541816377995No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT66040.24911965023661464No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC64910.24485700328374707No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT63320.23885911951820774No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT63100.23802922365127777No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT62320.23508686557761693No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA60070.2265992942112877No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT59790.22554306310792227No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC56320.21245334193407234No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA54290.20479566643467306No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG53820.2030227070825954No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC53780.20287181692497178No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC51790.19536503158319612No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA50180.189291702738845No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATTACGT48330.18231303294875204No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT46400.17503258284341183No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA43970.16586600576777627No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA42900.16182969405134415No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC40900.1542851861701626No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA40230.1517577760299668No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA39940.15066382238719547No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA39510.1490417531927414No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC39350.14843819256224688No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA39300.14824957986521736No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG39290.14821185732581146No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA38540.14538266687036838No Hit
CAACGGAATCCCAAAGATGATCTATTCCAATTACG36890.1391584478683936No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA36150.13636697995235644No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA34160.1288601946105808No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA33830.12761535081018585No Hit
TAGCTTATCAGACTGATGTTCTGGCTTCCAGCCGT33730.12723812541612678No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC33210.12527655336701957No Hit
TAGCTTATCAGACTGATGTTCTGGCTTCCAATTAC33090.12482388289414867No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG32680.12327725877850645No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC32620.123050923542071No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA32110.1211270740323697No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA31880.12025945562603384No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA30750.11599680867316625No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA30550.11524235788504811No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT29790.11237544489019911No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC28980.1093199191983206No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC28810.10867863602842015No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACGCCGT28540.10766012746446064No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT27640.10426509891792894No Hit
TGAGGTAGTAGTTTGTACAGTTACGCCTTGGCCGT27580.1040387636814935No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC27040.1020017465535745No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC26560.10019106466209093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)