FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209370
Sequences flagged as poor quality0
Sequence length35
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC869393.9350131485446074No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA728223.296052720911391No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT516392.337272616175652No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA472252.1374871569723495No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA272861.2350126959268932No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA271201.227499241865328No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA250521.1338978984959514No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC198800.899804016529599No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT162080.7336027917460634No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA154780.7005616985837592No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA154660.7000185573262967No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT113770.514943173845938No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC108630.4916786233179594No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA107800.48792189628717686No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC89460.4049118074383195No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT86650.39219324965940516No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA80820.36580563690101703No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT80430.3640404278142638No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT80350.3636783336426221No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT76200.34489469848870946No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC75560.34199794511557596No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA74950.33923697705680805No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62600.28333868930962225No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC58540.26496241009880644No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT58190.2633782480978741No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT57840.26179408609694166No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG56120.2540090614066453No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT52710.23857479734041828No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA52610.2381221796258662No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG51490.23305286122288255No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA50270.22753092510534675No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC49900.22585623956150394No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT42380.19181938742718513No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG40330.18254072427886683No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGACGC40260.18222389187868035No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA38740.17534410261748826No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT38110.17249261101580993No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC37970.171858946215437No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37920.17163263735816092No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC36860.16683488958390852No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT36590.16561282175461783No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC35900.16248975952420824No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA35590.16108664460909672No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT35350.16000036209417165No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA34700.1570583469495829No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC34240.1549763054626432No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT34180.15470473483391192No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA33660.15235112271824094No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG32690.14796073088708547No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA32290.146150260028877No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC31110.1408093709971621No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30600.1385010206529463No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC30080.13614740853727533No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCTAC27440.12419830087309956No Hit
CGCCTTGGCCGTACAGCAGCAGTTACCATAGACTA27110.1227046624150776No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA26790.12125628572851085No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA26410.1195363384132128No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25730.11645853795425845No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA25690.11627749086843761No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC25530.11555330252515422No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA25390.11491963772478128No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC25010.11319969040948326No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC24960.1129733815522072No Hit
TGGCTCAGTTCAGCAGGAACGCCTTGGCCGTACAG24610.11138921955127479No Hit
TCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24300.10998610463616326No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG23840.10790406314922353No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA23820.10781353960631311No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT23450.10613885406247028No Hit
TCGATCCCCGGCATCTCCACCACGCCTTGGCCGTA23090.1045094302900827No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA23060.10437364497571705No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA23040.10428312143280663No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC23010.10414733611844101No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG22730.10288000651769509No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22600.10229160348877735No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC22540.10202003286004609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)