FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4813985
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1441712.994836917854958No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN897561.8644844136406742No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT673481.3990072673678875No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA584201.2135476118018649No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN475100.9869162450651592No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT451670.9382455491656082No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN368850.7662051294301914No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA359420.7466163687672479No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA354980.7373932407350667No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN353410.73413190942639No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA328140.6816390163243135No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC313080.6503551631340772No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT310990.6460136456594693No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG293410.6094950441266435No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA282680.5872058180488722No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT268770.5583108381102143No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC258440.5368525244677745No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT255920.5316177761251852No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA255330.5303921802830711No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA254990.5296859047130392No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG249530.5183439499707623No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT220100.45720956754123665No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA207150.4303087774473747No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA202230.4200885544927955No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA186990.38843079070665987No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA185780.38591728058978164No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT172030.3573546656252564No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA165660.3441223850926No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA156660.32542685529763804No Hit
TCACTCCTCTCCTCCCGTCTTCCGCCTTGGCCGTA147340.30606659555441074No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAT143380.2978405624446275No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT142860.29676037627869634No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA141560.2940599108638685No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC140290.29142176388169055No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC136410.2833619132589736No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA135120.280682220655029No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA131990.2741803308485589No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC131530.27322478154792756No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG127910.2657050240081762No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC124900.25945240793230556No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGAA119120.2474457232417633No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA117890.24489066750311853No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG116660.2423356117644737No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGC112490.2336733496261413No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA111850.2323438897296107No Hit
CGCCTTGGCCGTACAGCAGTTTTCCTCTTAGAGGG111710.23205307037724465No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA109190.22681832203465527No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC105020.21815605989632292No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG104390.21684737281067556No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC97380.2022856323814885No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA92350.19183690850719312No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC89210.1853142458898397No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC88690.18423405972390858No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG87310.1813674118220144No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG85840.1783138086221706No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT84300.17511479574614378No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG84060.1746162482849448No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC82280.170918687947719No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA82120.17058632297358634No Hit
TATTGCACTTGTCCCGGCCCACTATTCCAATTACG81560.16942304556412205No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA77390.16076078342578964No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC73050.15174538350244132No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA73050.15174538350244132No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT70080.1455758586701039No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA69700.1447864918565388No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT68100.14146284211521223No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT66580.138305374860952No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA65500.13606191128555656No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG65440.13593727442025683No Hit
CATTGCACTTGTCTCGGTCTGACGCCTTGGCCGTA65250.1355425910134743No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC65040.13510636198492518No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG62430.1296846583443862No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCGC62290.12939383899202012No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA61660.1280851519063728No Hit
TCACTCCTCTCCTCCCGTCTTACGCCTTGGCCGTA60810.12631946298129304No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC60180.1250107758956457No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC56980.11836347641299257No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA54370.1129417727724536No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC53850.11186158660652246No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG53790.11173694974122271No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA53740.11163308568680626No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT53640.11142535757797335No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC53220.1105528995208751No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC52820.10972198708554348No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC52610.10928575805699436No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC51630.10725002259043184No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA49810.10346937100967285No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA49530.10288773230494072No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)