FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2761650
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN533721.9326127496243188No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC445731.6139988774826646No Hit
CGCCTTGGCCGTACAGCAGGTAGTAAGCTAGAGAT385151.39463726395452No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN296591.0739594083247335No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT272930.9882859884489346No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA241700.875201419441276No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA240050.8692267303966831No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT233770.8464867017905963No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT230700.83537015914399No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN219840.7960458421595784No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206780.7487552731157098No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA198490.7187369869462097No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT191730.6942588669816957No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA179980.6517118389368675No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA163130.590697590208752No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA157790.5713613238462513No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC156240.5657487371679975No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA141680.5130266326290442No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA138770.5024894537685805No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA132520.4798580558723951No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA127630.4621512501584198No Hit
CGCCTTGGCCGTACAGCAGGTAGTAAGCTAGATCG119240.4317708616225807No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT116760.4227907229373744No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA115760.4191696992739848No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC99250.3593865985914218No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC95730.34664059529629027No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA93260.3376966668477178No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC93260.3376966668477178No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA92320.3342929046041316No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA90490.3276664313001285No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA85540.3097423641663498No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA82680.2993862364890555No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC78680.2849021418354969No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT78220.28323647095033766No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC75030.27168540546412473No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC73790.2671953361215216No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA69520.2517335650788478No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT68220.24702623431644127No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC67540.24456393822533629No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA65870.2385168287074756No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA65830.23837198776094004No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC64270.2327231908460522No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT63950.2315644632737675No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA63390.22953669002226929No Hit
CGCCTTGGCCGTACAGCAGGTAGTAAGCTAGAGAA62540.2264588199083881No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT61620.22312747813806963No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC60900.22052034110042912No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA58930.2133869244835515No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA58530.21193851501819563No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA57960.20987453153006352No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCCTTG52820.1912624699002408No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG52350.18956058877844767No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC52330.18948816830517987No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC50040.1811960241160176No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA45610.16515488928720148No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA45370.16428584360798798No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG45170.16356163887531006No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA44820.16229428059312367No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT44200.1600492459218221No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC41750.15117773794651748No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT40610.1470497709702533No Hit
TCACTCCTCTCCTCCCGTCTTCCGCCTTGGCCGTA39470.1429218039939891No Hit
CGCCTTGGCCGTACAGCAGGTAGTAAGCTAGATGC38140.13810584252168087No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG37590.13611427950681657No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG37530.13589701808701318No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA37380.13535386453750475No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC36470.13205873300382018No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC35850.1298136983325186No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC35690.12923433454637626No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA34930.12648235656220014No Hit
TGAGGTAGTAGGTTGTGTGGTTCGCCTTGGCCGTA33010.11952999112849202No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA32950.11931272970868866No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC32750.11858852497601072No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC32070.11612622888490577No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT31910.11554686509876343No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG31710.11482266036608549No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC31680.11471402965618381No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA31100.11261383593141781No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT30310.10975322723733999No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA29500.10682019806999439No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG29400.10645809570365543No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC29380.10638567523038762No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA28650.10374232795611318No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGG27990.10135245233827603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)