FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246812
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1695923.9933955164485737No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1043532.4572079008913037No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA970712.2857381018985534No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA567381.336013932333242No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA552711.3014703735413766No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN550821.297019976396412No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT412220.9706575190990324No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA402780.9484290804490523No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT394590.9291440261542069No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA391220.9212086619327627No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN343150.8080178731716873No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA334580.7878380300328811No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA316410.7450529950466374No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA306910.7226832739476106No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAG279930.6591532660263746No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT260940.6144373709031622No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA260770.6140370706308639No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC257360.6060075181100553No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC257080.605348200014505No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA243540.5734654606796816No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC229950.5414649859706527No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN216650.5101473764320154No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC197480.46500763396166345No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA188220.44320304265882265No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA180610.4252837187047602No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN167410.39420157991453353No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA161480.3802381645337726No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC144890.34117356737241955No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT138370.3258208745760349No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC135230.31842709307593553No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC128420.3023915351091595No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC115140.27112101972020425No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA110410.25998325332037303No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA109090.2568750394413504No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT108360.2551561029779515No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA97010.22843017303332477No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC95220.2242152466367713No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA92210.21712757710960598No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA91770.21609150581659844No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA90630.21340713928471525No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA84410.19876085873356295No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGAGAC83400.19638260417461378No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC83390.19635905709977272No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC82810.19499332675899003No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA75270.17723883232881513No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC70410.16579495395604987No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT68450.161179727287198No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC67090.15797732510881105No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT66650.15694125381580348No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC66610.15684706551643915No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA64550.15199636809917652No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA63400.14928845449245223No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC62460.14707502945739062No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT62150.14634507013731712No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG61350.14446130415003067No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA60820.14321330918345337No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA60410.14224787911496908No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA59780.14076441339998097No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA59040.139021929861741No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGCTCA58940.1387864591133302No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA58920.13873936496364803No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG58490.13772684074548155No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT58150.1369262402008848No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT57870.13626692210533456No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA57020.13426542074384268No Hit
CGCCTTGGCCGTACAGCAGATCCTCGCTCAGCTCT55840.13148686591259515No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA54030.1272248453663595No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA52630.12392825488860822No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC52500.12362214291567417No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC51850.12209158305100391No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA51400.12103196468315527No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC50990.12006653461467097No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG50210.11822986277706667No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC49810.11728797978342341No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC48910.11516874304772615No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT48720.11472134862574562No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA48420.11401493638051319No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA47810.11257856481520725No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47680.1122724528422732No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG45360.10680953147914247No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA45300.10666824903009597No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT45200.10643277828168518No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA44890.10570281896161167No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA44700.10525542453963113No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA44140.10393678834853062No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA43520.10247686970838361No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA43500.10242977555870143No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG43040.10134661011601173No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC43010.10127596889148847No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT42830.10085212154434904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)