FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308347
Sequences flagged as poor quality0
Sequence length35
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1007703.045932001691479No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA448761.356447797041846No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT390781.1811941129512715No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA388791.17517902444937No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN376321.1374864849424804No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT284820.8609133201565616No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA273010.8252157346251768No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT271370.8202585762618009No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC269780.8154525507753569No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN265680.803059654866917No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT238730.7215990342004632No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA200870.6071612197874044No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC191090.5775996290594668No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC185100.5594939104029898No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN183940.5559876276581628No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN182430.5514234147748105No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA179410.5422949890081059No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA165950.5016100185379586No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC164500.497227165106925No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA154540.46712149602203157No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA139620.4220234455454642No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT127170.3843913591893474No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA122290.3696407904007651No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA118280.357519933670803No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC114170.3450968111869765No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA88150.26644726203146163No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA87270.263787323397455No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA83050.2510317085843776No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA80920.2445934480270661No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA79810.24123829815917136No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC77880.23540456910958857No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA75020.22675976854906696No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC72190.21820564771470466No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC71130.21500163072374212No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT70760.21388324743444384No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC69020.20862382331720344No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA67910.2052686734493087No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT66550.20115785919675291No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA64690.19553571617487525No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA64560.19514277069485153No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA63110.19075991726381786No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA62750.18967176054990603No Hit
CCGCCGGTGAAATACCACTACTCGCCTTGGCCGTA61110.18471460218653002No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA58310.17625116107832703No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT57290.17316805038891023No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGCTTA52770.1595056383142397No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA50640.15306737775692816No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG49050.14826135227048431No Hit
CCCGGGTTTCGGCACCACGCCTTGGCCGTACAGCA48760.1473847815842776No Hit
CGCGACCTCAGATCAGACGCGCCTTGGCCGTACAG44710.1351430185527697No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC44420.13426644786656297No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA44300.1339037289619257No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC44070.1332085177280376No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC42830.12946042238011912No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG39700.11999950428416366No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA37200.11244286043755386No Hit
GTTCGCGCTTTCCCCTGCGCCTTGGCCGTACAGCA37070.11204991495753014No Hit
CGCCTTGGCCGTACAGCAGTCCTACGCTTAGCTTG35050.10594414672946942No Hit
TCCGGGTGCCCCCTCCACGCCTTGGCCGTACAGCA33920.1025285437108018No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT33770.1020751450800052No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA33600.10156129329843575No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA33330.10074517576300188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)