FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2917703
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC526071.8030279298475547No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT515531.7669036224728836No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN330311.1320891811126765No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG301151.0321475489451806No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN297901.0210086496123836No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA270520.9271677069256192No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT254200.8712332955067736No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT225950.7744105551524607No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCCTT186120.6378990596369816No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCTCG183030.6273085368867222No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCTCC178060.6102745892916448No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCCTA176940.6064359532138809No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGCGGCATGTC163500.5603723202807139No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCGAT160620.5505015417950353No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA147380.5051233795900405No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA143880.4931276418470283No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN137770.4721865111013698No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT134910.46238427968850837No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC128070.43894118078502165No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN119210.4085748275269964No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC118870.4074095272891038No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT113740.3898272031114887No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA107370.3679949604192065No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT106710.36573290701623845No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC102210.3503098156323656No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA102210.3503098156323656No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA98690.3382455308165362No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC98560.33779997484322427No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA88000.30160712039573595No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG83200.2851558229196049No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC83070.28471026694629303No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCCTG76470.26208973291661286No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA76300.26150708279766655No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT71480.24498723824871826No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA71110.24371911740159982No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA70890.24296509960061047No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG70320.2410115080253199No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA66890.22925568503716792No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG66190.2268565374885655No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA66040.2263424344424364No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA64800.2220925159277692No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAGAA61960.212358831587725No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA60160.20618959503417586No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCGAA58830.20163121469183123No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA57440.1968671931310349No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC55670.1908007771867116No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA55480.19014957999494808No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC55240.18932701512114153No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC54040.18521419075210876No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC53660.18391179636858174No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA52950.18147837528357066No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC52340.1793876895626457No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAGAT51230.17558332702129037No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA50990.17476076214748382No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA50380.17267007642655885No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA48470.166123830972515No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCTCA48290.1655069073171601No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC48210.16523271902589126No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC47930.16427306000645028No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA47600.16314203330496627No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAAGG44780.15347689603773929No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC44630.15296279299161017No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC44400.15217450165421223No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCAGC44280.15176321921730895No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC43030.14747902716623318No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC42380.14525124729967376No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG40600.1391505578189418No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT40140.13757397514414593No Hit
TCAGTGCACTACAGAACTTTCGCCTTGGCCGTACA39020.13373533906638202No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC38440.13174747395468284No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC37400.12818302616818777No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT37030.12691490532106936No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGTTCC36510.1251326814278218No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC36140.12386456058070339No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCGAC35410.12136259242287512No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG35220.12071139523111159No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT35120.12036865986702554No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCAGT34970.11985455682089644No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT33680.11543327062418622No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC33450.11464497928678828No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGAATGC33420.11454215867756246No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGATCG33320.11419942331347638No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA33300.11413087624065918No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA33270.11402805563143337No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC33080.11337685843966984No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGAAGC32710.1121087375925514No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCAGG32640.11186882283769116No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGTCTT32080.1099495047988092No Hit
AAACCGTTACCATTACTGAGCGCCTTGGCCGTACA31940.1094696752890887No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA31440.10775599846865838No Hit
CTGACCTATGAATTGACAGCTGTATTCCATTTACG31240.10707052774048627No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT31180.10686488652203463No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA30760.10542539799287318No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC30740.10535685092005595No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA30280.10378026824526004No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA30150.10333471227194817No Hit
TGACCTATGAATTGACAGCCACGCCTTGGGGCATT29650.10162103545151785No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT29360.10062710289566827No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG29210.10011299984953917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)