FastQCFastQC Report
Wed 8 May 2024
EGAF00002406246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3564174
Total Bases124.7 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT754662.1173489285315474No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA483531.35663971512053No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC477381.3393846652828958No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN440061.2346759726096426No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA402211.128480259381276No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN339950.9537974296428849No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT339060.951300357389959No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA336340.9436688556731517No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA335140.9403020166804426No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA329750.9251792982048577No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA281680.7903093395552517No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA248600.697496811322904No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA217030.6089208888230484No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT213130.5979786620967439No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA180030.5051100198811842No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT172270.48333779439499863No Hit
CGCCTTGGCCGTACAGCAGCCCTAGATTCAGAGAC166390.466840283330724No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT165230.46358567230443853No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAATGCA159340.447060104248558No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT159230.44675147734089304No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA154000.4320776707310025No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN152390.4275604950824511No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN148790.4174599781043237No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC138940.38982384137250314No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA132820.37265296250968666No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG131950.3702120042399726No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC124100.34818726582933385No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA119300.33471990985849737No Hit
CGCCTTGGCCGTACAGCAGCCCTAGATTCAGAGAG116040.32557333059497096No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT109210.3064104053281349No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT107640.30200545764600717No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA102170.2866582832375748No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT96440.2705816270473888No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA95780.26872986560139883No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC79860.2240631349647913No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAGCATG79380.22271639936770762No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA79370.22268834237610174No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT78640.22064018198887037No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT77330.21696471608849624No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA76250.21393456099505803No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG71960.20189811159612298No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAAAC69990.19637088424975885No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT66160.1856250564646956No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC63340.1777129848318292No Hit
TAGCTTATCAGACTGATGTTGACGCGGACAATTGT62860.17636624923474556No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT62000.17395334795663736No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA61580.17277495430918918No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC60760.1704742809975046No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA60700.17030593904786914No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA60500.16974479921575097No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT59950.16820166467742595No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC59850.16792109476136688No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA59490.16691104306355414No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC55620.15605298731206724No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC53810.15097467183139768No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAATGC52660.14774811779671812No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC51740.14516687456897448No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA51000.14309065719013717No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA50410.14143529468538854No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA50330.14121083875254126No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA49100.13775982878501442No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA48370.13571166839778306No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC48100.1349541296244235No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC47680.1337757359769753No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC46610.13077363787514304No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA45650.12808016668097574No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTTG45560.12782765375652255No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC45490.1276312548152812No Hit
CTGACCTATGAATTGACAGCTTATGAACCGGCATG44930.12606006328535027No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC44870.12589172133571483No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA44180.12395578891490706No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG42800.1200839240732916No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA42060.1180077066944543No Hit
GTCAGTTTGTCAAATACCCCACGCCTTGGCCGTAC41720.1170537689798534No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC41480.11638040118131157No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT41010.11506172257583384No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA40570.11382721494517384No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA40140.11262076430611974No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT37710.10580291534588379No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT37570.10541011746340107No Hit
TGTCAGTTTGTCAAATACCCCCGCCTTGGCCGTAC37300.1046525786900415No Hit
CGCCTTGGCCGTACAGCAGCCCTAGATTCAGCTCA36980.10375475495865241No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA36980.10375475495865241No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG36930.10361447000062286No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG36500.10240801936156876No Hit
TAGCTTATCAGACTGATGTTGACATAAGTGGCCAC36380.10207133546229785No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA36290.10181882253784467No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGATGCA35720.10021957401630784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)