FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338337
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN384581.152010716713142No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN302220.9053010525899572No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGATGCA270640.8107030536461717No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165870.4968641572136066No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN154580.4630449232656859No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT136960.41026415248071124No Hit
CTGACCTATGAATTGACAGCCCGCCTTATAGCATG134670.40340444958073435No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCTAC125130.3748273466699138No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGGATG102930.3083271700849854No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATGATG100880.3021863880129537No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATCGTAC97830.29305010249115054No Hit
CTGACCTATGAATTGACAGCCCGCGAACGCTACGT83570.2503342232974083No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGCGATGC75600.226460060802729No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCTACG66120.19806268809889477No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAATCGT65480.19614556589104098No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGATGCA65010.19473767926964833No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAGCA61930.18551152864435197No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATTCGT58750.17598582767407844No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCTCGTA58200.1743383007767041No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGATGAC53880.161397725873691No Hit
AGCAGCATTGTACAGGGCTATGACGCCTGCGTCGT52960.1586418626999012No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT51960.15564635925012965No Hit
CTGACCTATGAATTGACAGCCACGCCTTCGAGCAT48160.14426344614099773No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCAGCAT47400.1419868635191714No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCAGCATG45080.1350372955157014No Hit
CTGACCTATGAATTGACAGCCACGCCTGATCTACG44630.1336893189633042No Hit
CTGACCTATGAATTGACAGCCCGCCTTAGCTACGT41930.125601459648921No Hit
CTGACCTATGAATTGACAGCCACGCCTTATCTACG41330.12380415757905809No Hit
TGACCTATGAATTGACAGCCAGCGCCTATTGATGC40130.12020955343933222No Hit
CTGACCTATGAATTGACAGCCCGCGAATATCGTAC39220.11748364530004012No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCAGCA38970.11673476943759722No Hit
CTGACCTATGAATTGACAGCCCGCCTTAATCGTAC38790.11619557881663833No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTAAAGCA38740.11604580364414976No Hit
AGCAGCATTGTACAGGGCTATGACGCCTGCGGATG38040.11394895122930969No Hit
CTGACCTATGAATTGACAGCCACGCCTGTAGATGC37980.11376922102232338No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGTCGTAC37170.11134286322800843No Hit
TGACCTATGAATTGACAGCCACGCCTGCGGATGCA36680.10987506653762037No Hit
CTGACCTATGAATTGACAGCCCGCCTTATAGCAGT36590.10960547122714093No Hit
CTGACCTATGAATTGACAGCCACGCCTGCGAGCAT35240.10556154156994935No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGCGATG35020.10490253081099962No Hit
TAGCACCATCTGAAATCGGTTACGCCTTATAGCAT34590.10361446432759784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)