FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2941624
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1289494.383598991577442No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT619992.107645300691047No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT454821.5461527373994775No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA379611.2904776409221572No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN347151.1801304313535652No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA337861.1485492367481365No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA334721.1378748609611562No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA317901.0806955613633829No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA309341.0515959891542903No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT290070.9860879568564848No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA284530.9672548225062074No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN218690.7434328792530929No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA192100.6530406333372314No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA181200.6159862715289242No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA168390.5724388976973264No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN167350.568903435653231No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA160780.5465688340862055No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA159280.5414696099841447No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA157540.5355545100257545No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150240.5107382860623928No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT136440.463825424323435No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC129350.4397230917343617No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCGC116190.3949858989456164No Hit
TCCCTGAGACCCTAACTTGTGACGTTCCAATTACG115780.39359211102438657No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT110200.374622997364721No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT105310.3579995267920033No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA99640.3387244596862142No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC98780.33580090453436606No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC89020.3026219530436249No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG88670.3014321340864774No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA85980.29228752553011533No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA85400.2903158255439852No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT83180.2827689738729355No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG75020.2550291947577257No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA73980.2514937327136303No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACGCC72030.2448647413809515No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT71880.2443548189707454No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC69460.23612807075275424No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCG66040.224501839800056No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA64990.22093238292861359No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT62760.2133515364302168No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA59760.20315308822609549No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCCTTGGC56280.19132288830931488No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC53370.18143039355131724No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA51340.17452944359986183No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT50470.1715718936206667No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA50060.17017810569943675No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTG48820.16596274710839998No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC48720.16562279883492928No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC48430.16463694884186422No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT46980.15970769887653893No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA45800.15569630924958458No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC45300.15399656788223104No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCGCTCCA45130.15341865581733086No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA45100.15331667133528962No Hit
GCTAAACCTAGCCCCAAACCCGCCTTGGCCGTACA44200.15025713687405326No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA43990.14954324549976475No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT43930.14933927653568232No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGGGT43650.14838742136996436No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG41840.14223435762014452No Hit
TAGCTTATCAGACTGATGTTGACCGCCAACCGAAC41520.14114652314503826No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA40860.13890286454013157No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA40070.13621727317971297No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA39820.13536740249603618No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAGG38300.13020018873928144No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC37160.12632477842171536No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA37150.12629078359436827No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA36170.12295929051435532No Hit
CCCAGTGTTTAGACTATCTGTTCGCCTTGGCCGTA35860.12190545086659614No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA35470.12057965260006039No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT35290.11996774570781309No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTCGC34920.11870993709597148No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA34120.11599035090820581No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA33880.1151744750518761No Hit
CCCAGTGTTTAGACTATCTGTTCCGCCTTGGCCGT33770.11480053195105833No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA33520.11395066126738156No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT33500.1138826716126874No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA33410.11357671816656378No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC33090.11248888369145751No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT32860.11170700266247488No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT32810.11153702852573953No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG32680.11109509577022761No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT31760.10796757165429707No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC31370.10664177338776132No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC30300.10300432686162474No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT29860.10150855445835362No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCGC29520.1003527303285532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)