FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5056292
Sequences flagged as poor quality0
Sequence length35
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC3399336.722970113276686No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA1894203.7462235171544687No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1177242.3282674339219334No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT1175862.325538161166325No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1008281.9941095174092003No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT976071.930406709106199No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT932301.8438412971402758No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA644881.275401025099025No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN626131.2383185148326086No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA581161.1493798222096352No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT525571.0394375957717632No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA514511.0175638590492797No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA463200.916086333621555No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT451680.8933028393138688No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA430620.8516517637826296No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT353660.6994453643104472No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC352610.6973687437355279No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN345920.6841377040724705No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT332040.6566867578059178No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC315530.6240343714326625No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT283870.5614193167641426No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA269110.5322279646824194No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN253700.5017510855781272No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT222180.43941291365292984No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA218700.4325303997474829No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA218620.43237218103701286No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA214510.4242436947866144No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA211480.41825116112756144No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA190190.376145206803721No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA189500.3747805704259169No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT170640.337480509432604No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC169260.33475123667699574No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA154300.30516433781909746No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA154210.30498634176981865No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT152330.30126820207377264No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA146040.28882825596306544No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA141630.28010644954840425No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT137840.2726108381398859No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA130770.25862825960209573No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA126730.2506382147233585No Hit
TAGCTTATCAGACTGATGTTGACGCCTGTTAATGC123200.24365681412386783No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA116920.23123664535196936No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA116720.23084109857579427No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT115950.22931824348752008No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC114140.22573854516313535No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG113410.22429479943009623No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT109880.21731339883060552No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA106640.21090554105656872No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC100980.1997115672908131No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT97940.19369925629295145No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC94420.1867376330322695No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG93090.18410724697070502No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA89150.17631497548005534No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT88450.17493056176344246No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC87210.1724781717511568No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC86460.17099487134050012No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA86220.17052021520908997No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA85390.16887869608796327No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA84280.16668341148019142No Hit
TGTGCAAATCCATGCAAAACTGCGCCTTGGCCGTA81990.16215440089298638No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA78880.15600364852346343No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA78770.15578609779656713No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA76550.1513955285810234No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA75750.14981334147632297No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC73040.14445368265915023No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA71440.14128930844974932No Hit
TCTCACACAGAAATCGCACCCGTCCGCCTTGGCCG70760.13994444941075398No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC70300.13903469182555123No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC70180.13879736375984614No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG68360.13519788809665265No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA67240.13298282615007204No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA65340.1292251317764085No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT65140.12882958500023337No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC64230.1270298471686366No Hit
ACTGGACTTGGAGTCAGAAGGCCGCCTTGGCCGTA61780.12218439916049152No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC60580.11981111850344085No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA59460.11759605655686023No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC59090.11686429502093629No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC58780.11625119751786488No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG56990.11271105387109764No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC56720.11217706572326124No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA56090.11093109337830964No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC55220.1092104649019479No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC54360.10750961376439494No Hit
CGTACCGTGAGTAATAATGCGACGCCTTGGCCGTA53950.10669874287323597No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC53350.10551210254471062No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA52370.10357392334145259No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA52180.10319815390408622No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA51870.10258505640101481No Hit
TAGCACCATTTGAAATCAGTGACGCCTTGGCCGTA51400.10165552147700331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)