FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3164802
Sequences flagged as poor quality0
Sequence length35
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT863832.7294914500180423No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA545041.7221930471479734No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN478541.5120693174486113No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC399751.2631121946965402No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA394671.2470606376007092No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT378731.196694137579539No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA368631.1647806087079065No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA280540.8864377613512631No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN264910.8370507854835785No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA257100.8123730963264053No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA200660.6340365052853227No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAATGC179760.5679976188083804No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN167440.5290694330956566No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA151470.4786081404144714No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC150090.4742476780537929No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT125670.39708645280178667No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC125590.3968336723750807No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA124250.39259960022775514No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN123600.3905457592607689No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT122380.3866908577535024No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT122050.3856481384933402No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA120740.38150885900602943No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA120340.38024495687249943No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA116000.366531618723699No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC114730.36251872944974123No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA108460.3427070635066586No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA105240.3325326513317421No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA94670.29913403745321193No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA85560.2703486663620662No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT83420.26358678994768076No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA79190.25022102488560105No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG76310.2411209295241851No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT76090.24042578335074358No Hit
CGCCTTGGCCGTACAGCAGCCCTAGATTCAGAGAC76060.24033099069072883No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC71340.22541694551507485No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC70150.22165683666782315No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA68090.21514774068014364No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA67230.21243035109305414No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA66780.2110084611928329No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT65500.20696397436553693No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA64710.20446776765181518No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC61410.19404057505019273No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG60820.19217631940323598No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC58530.18494047968877675No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC55950.17678831092750824No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA54880.1734073727203155No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC54460.17208027548010904No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA53310.1684465568462103No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT52910.1671826547126803No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA52910.1671826547126803No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC52460.16576076481245905No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT52140.16474964310563503No Hit
CTGACCTATGAATTGACAGCCCGCAGGTTAATGCA51510.1627589972453253No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC50850.1606735587250008No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA49960.15786137647789655No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA48160.1521738168770116No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA47630.15049914655008434No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC47160.14901406154318658No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG44310.14000875884178537No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT42300.13365765062079712No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC40100.12670618888638213No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC39880.12601104271294067No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC39110.12357803110589541No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC38690.12225093386568892No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA37930.11984951981198191No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC37890.11972312959862894No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT37650.11896478831851093No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA37500.11849082501843716No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC37100.11722692288490717No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA36980.11684775224484818No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAGCAT36690.11593142319803894No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT35740.11292965563090519No Hit
TGACCTATGAATTGACAGCCACGCCTGTTAATGCA35670.11270847275753743No Hit
TCTCACACAGAAATCGCACCCGTCCGCCTTGGCCG33940.10724209603002022No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAT32580.10294482877601821No Hit
ATCACATTGCCAGGGATTACCACCGCCTTGGCCGT32120.10149134132245873No Hit
TGACCTATGAATTGACAGCCAGTCGTTCCAATTAC31980.10104897557572322No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA31830.10057501227564948No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)