FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257848
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1060873.2563520458904165No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT674262.0696484304976783No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA487881.497552985897439No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA473971.454856089050195No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA419781.2885192924900117No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT395321.2134390554746568No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA333611.0240195368230807No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT321260.9861110770054342No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC299450.9191650439185622No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA294440.9037867942273551No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT201620.6188747909663066No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA196660.6036500168209199No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT181740.5578529139481031No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA150410.46168513693702096No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA150120.4607949787712625No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC148140.4547173471567734No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGCT145060.44526325353423485No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA143510.44050551161380147No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT124450.38200063354705316No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA119630.36720559092996363No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA116520.3576594119799328No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC115250.3537611331160938No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC111960.3436624422011095No Hit
CTGACCTATGAATTGACAGCCCGAAGGTCGGCATG108990.3345459947793758No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA107320.32941991154897343No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT105490.32380270657194565No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC104310.3201806836905835No Hit
CTGACCTATGAATTGACAGCCGCCTTGCGGCATGT103220.31683491679169806No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA103030.31625170971758043No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA98360.30191709373795217No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA94650.29052920823807615No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT91200.2799393955764664No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA87680.2691347171507081No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC87330.26806038832996504No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA87280.26790691278414464No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT86220.26465323121275147No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT82220.25237518754711696No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA81530.250257225014795No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC80160.24605199505931524No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT79480.24396472763615734No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC77000.236352340563464No Hit
TGACCTATGAATTGACAGCCAGCGCCTACCGGCAT72720.22321483384123508No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA71840.22051366423479546No Hit
TGTCAGTTTGTCAAATACCCCACGCCTGTTAATGC71460.21934725008656022No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG71180.2184877870299658No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68170.20924855917157584No Hit
CTGACCTATGAATTGACAGCCCGAAGGTATTACGT68120.2090950836257554No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA65490.20102226991560074No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC63870.19604966223101877No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA56890.17462447603448658No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG55550.17051133140649902No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT55110.1691607466032792No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA54950.16866962485665382No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG54860.16839336887417708No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA54160.16624471123269102No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC53510.16424952913702542No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC53110.16302172477046198No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT52780.16200878616804712No Hit
CTGACCTATGAATTGACAGCCCGCCTTGATTACGT52590.1614255790939295No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51290.15743521490259826No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC51120.15691339804680882No Hit
CTGACCTATGAATTGACAGCCCGAAGGTCGGCAAC50830.15602323988105032No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGGT49530.1520328756897191No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA48860.14997630337572532No Hit
CGCCTTGGCCGTACAGCAGCGGGCTCCCGAGGGAG48690.14945448651993584No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA47640.1462315000577068No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT47600.14610871962105046No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA46470.1426401722855087No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA45600.1399696977882332No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA45050.13828146678420847No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA42970.13189688407807854No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC42960.13186618896891444No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG42930.1317741036414222No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC42820.13143645744061724No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC41480.12732331281262968No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTATTAC40080.12302599752965762No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCTAC39960.12265765621968859No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC39490.12121498608897653No Hit
TAGCTTATCAGACTGATGTTCTGGCTTCCAGCCGT39210.1203555230323821No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39180.12026343770488986No Hit
CTGACCTATGAATTGACAGCCGCCTTGTAATGCAC38550.11832964582755243No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT38010.11667210993269177No Hit
GTCAGTTTGTCAAATACCCCAACGCCTTGGCCGTA37800.11602751264024595No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC37050.11372537945293949No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC36710.11268174574136054No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG36290.11139255115646893No Hit
CTGACCTATGAATTGACAGCCCGAAGGTCGGCAGT35900.11019544189906957No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC35860.11007266146241322No Hit
TGTCAGTTTGTCAAATACCCCCGCCTTGGCCGTAC35650.1094280641699674No Hit
TAGCACCATCTGAAGCTAACCGTATTCCAATTACG35080.10767844294761451No Hit
CTCCTGACTCCAGGTCCTGTGTCGCCTTGGCCGTA34450.10574465107027707No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC34430.10568326085194889No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA33810.10378016408377555No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGGTCGGCA33780.10368807875628329No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)