FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481294
Sequences flagged as poor quality0
Sequence length35
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT51777320.867055657249807No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA2421569.759262707280959No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC424201.7095918500588805No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG320991.2936395284073552No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA300321.2103362197305116No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA233480.9409606439220826No Hit
GTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN223420.9004172822728785No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT207550.8364587187169275No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGCGGCATG192870.7772960398888644No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA184070.7418306738338948No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA175770.7083803853956847No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT170450.6869399595533621No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCCAT159020.6408752852342366No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA151890.6121402784192441No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC121670.49034898726229137No Hit
GTCCAGTTTTCCCAGGAATCCACGCCTTGGCCGTA120680.4863591335811073No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA113900.4590346810978465No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA98050.3951567206465658No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA90740.3656962858895399No Hit
GTCCAGTTTTCCCAGGAATCCCTACGCCTTGGCCG89980.36263336791206524No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG85280.3436916383145246No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA78700.3171732168779677No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC64450.2597435048003179No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT59530.23991514105140302No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT59410.2394315224233807No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT58830.23709403238793952No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC58590.23612679513189488No Hit
CGCCTTGGCCGTACAGCAGATACCGACAACTGTTG48910.1971148924714282No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC46070.18566925160823344No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA43950.17712532251317256No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA42420.17095918500588805No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA41980.16918591670313957No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG41650.1678559654760782No Hit
GNNCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT39730.16011806742772117No Hit
GNNGTCAAAAGGGTCCTTAGGGAGCGGAACCGGCA39700.1599971627707156No Hit
GNNACTGGGGAAACTTCCGAAAGATAAGGTTAATG39320.15846570378197827No Hit
GNNTGACCCCTTTGAAGGCTTTCTATTCCAATTAC39190.15794178360162078No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC37930.15286378800738648No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC35890.14464227133100713No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA33160.13363994754349948No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC32850.1323905994211085No Hit
GTCCAGTTTTCCCAGGAATCACGCCTTGGCCGTAC31800.12815893642591325No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC31330.1262647634661592No Hit
AGCAGCATTGTACAGGGCTATGCATAAGGTTAATG28110.1132876636142271No Hit
TCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27230.10974112700873012No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG27020.10889479440969108No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA26960.10865298509567992No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTGGCAT26160.10542886090886448No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25750.10377649726312159No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC25520.10284956155941213No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT25060.10099569015199328No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC24840.10010905600061903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)