FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600538
Sequences flagged as poor quality0
Sequence length35
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC520871.4466449180650225No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA491711.3656570212562678No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA379411.0537591882102064No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT314840.8744248776155119No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT304820.8465957031976887No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA284920.7913261851423316No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC251260.6978401561100036No Hit
CATCACCCCATAAACACCACGCCTTGGCCGTACAG249700.6935074702724981No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT219270.6089923228139795No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAC201000.558249905986272No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT176550.490343387571524No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA172780.479872730130886No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGCACA169840.47170728374481813No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA166160.4614865889486516No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA151760.42149256583321715No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC146810.4077446203872866No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC115170.31986886404198484No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA114870.31903565522707994No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC109460.30401012293162855No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAA104320.28973447856959156No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT100690.27965265190924243No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC97480.2707373175897602No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACTG96880.2690708999599504No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA95200.26440493059648307No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT94400.26218304042340335No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA92560.2570726930253201No Hit
GTTCGCGCTTTCCCCTGCGCCTTGGCCGTACAGCA85220.23668685068731396No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA84380.23435386600558025No Hit
TAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN84170.23377061983514685No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC81710.2269383075529268No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGCA79400.2205225996781592No Hit
ATCACCCCATAAACACCACGCCTTGGCCGTACAGC75850.21066296203511806No Hit
ATCCCACTCCTGACACCACGCCTTGGCCGTACAGC69550.19316557692211553No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC69480.19297116153197105No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACTT66700.18525009318051913No Hit
TCACCCCATAAACACCACGCCTTGGCCGTACAGTG66040.1834170337877284No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTGGCAT65730.18255605134566No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA65130.18088963371585023No Hit
CACATCACCCCATAAACACCACGCCTTGGCCGTAC64370.17877883805142453No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA62340.17314079173723482No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG60290.16744719816871811No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC59950.16650289484515926No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA57970.161003716666787No Hit
AACACCTCTTTACAGTGACCACGCCTTGGCCGTAC57390.15939284629130424No Hit
TATTGCACTTGTCCCGGCCTGTCGCAGGTTAATGC55770.15489351869081788No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA55450.154004762621586No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA55420.1539214417400955No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG53680.14908883061364717No Hit
GGCTCACATCACCCCATAAACACCACGCCTTGGCC52650.14622814701580708No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA50510.1402845908028189No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA49290.1368962082888724No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA49190.13661847201723742No Hit
CTGACCTATGAATTGACGATTATTCCATAATGCAC46040.12786977946073616No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA45560.12653664535688833No Hit
GCTAAACCTAGCCCCAAACCCACTCGCCTTGGCCG45000.12498132223573255No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC43170.11989874846481274No Hit
ACATCACCCCATAAACACCACGCCTTGGCCGTACA42670.11851006710663795No Hit
CTGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39700.1102612998390796No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT38160.1059841612559012No Hit
CGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37850.10512317881383282No Hit
ATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37590.10440106450758194No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA36970.10267909962344515No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGCAGT36920.10254023148762768No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT36700.10192921169003077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)