FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232144
Sequences flagged as poor quality0
Sequence length35
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1918605.935997901083615No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA912632.823605631432263No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT685432.1206666534659346No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA601581.8612413308317945No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT568141.7577805939339337No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA484741.4997475360008712No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN435731.3481144404457226No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA352071.0892769629075931No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA351501.0875134276195615No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA340921.0547797375364465No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA339561.0505720042176339No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN299420.9263819928815052No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC237050.7334141053121396No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC177200.5482429000688088No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT171760.5314119667935587No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA164280.5082694335400898No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA147080.4550539827433431No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA145560.45035122197525856No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA133180.4120484730878327No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT132740.41068714760233455No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN130710.40440648683969527No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC124770.38602859278546997No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA123390.38175898103549843No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGCA119030.36826948304283474No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114110.3530473889777188No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA113330.3506341301625175No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGATG112880.34924186546143987No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA102860.31824077145077695No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCCA102100.31588939106673464No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA100630.3113413263765476No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA83450.25818775401095995No Hit
CTGACCTATGAATTGACAGCCCGCCTTGCGGCATG77290.2391291972139855No Hit
TAGCTTATCAGACTGATGTTGACGCCTTGGCCGTA73880.2285789247013747No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA72850.2253921854966858No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCAATTAC72210.22341207569959753No Hit
TAGCTTATCAGACTGATGTTCTGCGGAACCGGCAT71790.22211262864525838No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT65320.20209495616531936No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA64660.2000529679370721No Hit
TGACCTATGAATTGACAGCCAGCGGGAACCGGCAT64610.19989827185917458No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCTAC61480.1902142973827899No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA60850.18826512680128113No Hit
CGCCTTGGCCGTACAGCAGCTAAGACGACAGAGAG57690.17848833467815792No Hit
CCCATAAAGTAGAAAGCACTCGCCTTGGCCGTACA54910.1698872327470558No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA52390.16209055042102086No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC51680.15989386611487608No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA50970.1576971818087313No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT50450.1560883425985971No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA50080.15494359162215543No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC49730.15386071907687282No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA49680.1537060229989753No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTATAGCA48350.14959110732690128No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA47340.14646624655337137No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT45520.14083530931790167No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC44200.13675133286140717No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA43900.13582315639402207No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT43660.13508061522011394No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG43390.13424525639946736No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT43070.1332552015009232No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG42580.13173917993752754No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC42520.1315535446440505No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGCGGCATG40670.1258297897618423No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA40590.12558227603720626No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC40270.12459222113866213No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG37980.11750714077095575No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA37370.11561984862060602No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC35820.11082427020578292No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA35190.10887509962427416No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC34790.10763753100109402No Hit
CTGCCAATTCCATAGGTCACAGCGCCTTGGCCGTA34300.10612150943769831No Hit
CGCCTTGGCCGTACAGCAGCTAAGACGACAGAGAC33780.10451267022756412No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT33740.1043889133652461No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG33590.10392482513155354No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC33530.10373918983807653No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA32880.10172814082540878No Hit
CGCCTTGGCCGTACAGCAGCTAAGACGACAGAGTC32410.10027399769317209No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)