FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3662685
Sequences flagged as poor quality0
Sequence length35
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN558291.524264303373072No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA555201.5158278694455023No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC532741.454506734813395No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT360990.985588441266448No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT351460.959569277729316No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT332630.9081589052839653No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA285490.7794555087319821No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA270050.7373006414693046No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN243520.6648674401429552No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA197660.5396587476127486No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT196640.5368739053453956No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC196180.5356179960875697No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTG186580.5094077159242468No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAC171380.4679081056656524No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA170770.46624266078027454No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA166570.4547756632088208No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA166420.45436612758126893No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165820.45272798507106127No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA158670.4332067868244198No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA155320.42406049114242694No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC144410.3942735998318174No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA142040.38780293691649703No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN138890.37920268873790675No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGAA134800.3680360172933244No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGAGTT115450.3152059213391269No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC109870.2999711959941955No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA97370.2658432270315356No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG95680.261229125627784No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT93320.2547857650876338No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC91760.25052659456109383No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA90810.2479328689199317No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA88760.2423358820100555No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGGCCGTACAG88200.2408069490005283No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA83870.2289850205518629No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA81350.22210482200899065No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA80830.220685098500144No Hit
TAGCTTATCAGACTGATGTTCTGGCGGACAATTGT77760.21230326932291477No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA72670.19840636036131962No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA67590.18453675377489465No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC66970.1828440065143467No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC66370.18120586400413904No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT65910.17994995474631317No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC63410.17312436095378117No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA63220.17260561582554873No Hit
TCCCCAGTACCTCCACCACGCCTTGGCCGTACAGC59240.16173927050783785No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG58740.16037415174933145No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT57630.15734358810544724No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGACAC54630.14915287555440887No Hit
TAGCTTATCAGACTGATGTTCTGCGCCTGTTAACA54370.14844301379998553No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC53540.1461769166608649No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT52670.14380161002106379No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC50690.13839573973737845No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC49890.1362115497237682No Hit
CGCCTTGGCCGTACAGCAGCGCCTTGTAATGCACT49510.13517405946730338No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA49310.13462801196390078No Hit
CAAAGTGCTTACAGTGCAGGTAGCGCCTTGGCCGT47900.13077837706491277No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG47740.13034153906219073No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA47630.1300412129353193No Hit
ACCGGGCGGAAACACCACGCCTTGGCCGTACAGCA46970.12823925617409085No Hit
CACTCCTCTCCTCCCGTCTTCTCGCCTTGGCCGTA46800.12777511579619868No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT46760.12766590629551816No Hit
TAGCTTATCAGACTGATGTTGACGCGGACAATTGT46590.127201765917626No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA46270.12632808991218192No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC44500.12149556950706926No Hit
CGCCTTGGCCGTACAGCAGCCTCTTACACAGACTG43860.11974821749618109No Hit
TAGCTTATCAGACTGATGTTCTGGCGGACAATTTG42090.11491569709106844No Hit
TAGCTTATCAGACTGATGTTCTGGCAAGTGGCCAC41190.11245848332575692No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA39820.10871805792744939No Hit
ATCACATTGCCAGGGATTTCGCCTTGGCCGTACAG38950.10634275128764827No Hit
ACAGCAGGCACAGACAGGCAGTCGCCTTGGCCGTA37660.10282074489070177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)