FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002406256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044192
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1269544.170367703482566No Hit
CCACTACGCCTTGGCCGTACAGCAGTCATCTTCTC625782.055652205905541No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA564841.8554677234550252No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN483861.589452964858984No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN412191.354021034152905No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT378571.2435812195814193No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAC331551.0891231564894723No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC305521.0036160662665166No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN248070.8148960380948377No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA239710.787433906928341No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC230730.7579351105317931No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA214160.7035035897867151No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT206910.679687746370794No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA200570.6588612019215608No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN198750.6528826039881848No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA169410.5565023493918911No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC158970.5222075348729647No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA155740.5115971660131818No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT130580.4289479770001366No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA126080.41416572936266827No Hit
CCACTACGCCTCCGCCTTGGCCGTACAGCAGTCAT121330.3985622457453406No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT120300.3951787535083201No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA107690.3537556106842144No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG104390.34291529575007096No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA96490.3169642387865154No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG95020.31213537122494245No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA92820.3049084946021801No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA84490.27754491175326657No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA81290.26703309121106683No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT81010.26611330691362434No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT79810.26217137421029946No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC79610.261514385426412No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA78810.25888643029086206No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT77770.25547008861464715No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA75480.24794756703913548No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT74580.24499111751164182No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAG71810.23589182285480023No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA70880.23283682500972344No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA70210.23063591258370036No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA65170.21407979522973583No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC58370.19174217657756146No Hit
CTGACCTATGAATTGACAGCTGTATTCCATAATGC58290.19147938106400647No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC58150.19101948891528522No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA56880.18684761013759973No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG56100.18428535388043857No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC55380.1819201942584436No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT54260.17824105706867371No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT53110.17446337156132072No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC51680.16976590175652523No Hit
CGCCTTGGCCGTACAGCAGTCATCTTCTCAGAGAA51080.16779493540486276No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG49740.16339311055281663No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG49630.16303176672167854No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT49540.16273612176892915No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC47120.15478655748389064No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCGGCAT46900.1540638698216144No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTATTAC46840.15386677318644817No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT46400.1524213978618957No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT45590.14976059328715138No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA45560.14966204496956828No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT45220.14854516403695955No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC44850.14732973478676772No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA44470.14608145609738152No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA42920.14098979302225353No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA42720.14033280423836605No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC41540.1364565704134299No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA41150.13517544228484932No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG41090.13497834564968306No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA39840.13087216575038632No Hit
TGACCTATGAATTGACAGCCACGCCTTGCGGCATG39270.12899974771630698No Hit
ATCCCACTTCTGACACCACGCCTTGGCCGTACAGC38030.1249264172562046No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT37610.1235467408100409No Hit
AGCAGCATTGTACAGGGCTCGTCATAAGGTTAATG37350.12269265539098717No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA37290.12249555875582091No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA36900.12121443062724033No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC36270.11914491595799476No Hit
CTGACCTATGAATTGACAGCTGTATTCCATTTACG36080.11852077661330165No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC35140.1154329293290305No Hit
TGACCTATGAATTGACAGCCACGCCTTGGGGCATG34970.11487448886272614No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC34920.11471024166675425No Hit
CACTACGCCTTGGCCGTACAGCAGTCATCTTCTCA34070.11191803933523246No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC34030.11178664157845497No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA33730.11080115840262374No Hit
TTTGTTCGTTCGGCTCGCGTGACGCCTTGGCCGTA33450.10988137410518127No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC33370.10961857859162628No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT32930.10817320326707383No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC31250.10265449748241898No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA31210.10252309972564148No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC31200.10249025028644711No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT30760.10104487496189465No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)