FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4914460
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1637313.331617308921021No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1200202.442180829633368No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1028392.0925798561795195No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT884491.7997704732564717No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA743231.512332992841533No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA623371.2684404797271724No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA592431.205483410181383No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT500871.0191760641047032No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN482630.9820611013214067No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN458860.9336936306328671No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA434290.8836983106994462No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA397930.809712562519585No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT355680.7237417742742845No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA348660.7094573971504499No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA348420.7089690423769854No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTCG295680.6016530809081772No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN288100.5862292093129255No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT286340.5826479409741864No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC265950.5411581333452709No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA262940.5350333505614046No Hit
TGAGATGAAGCACTGTAGCTCTCGCCTTGGCCGTA243770.4960260130309332No Hit
TGAGATGAAGCACTGTAGCTCCGCCTTGGCCGTAC242010.49244474469219407No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCG225870.4596028861767112No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT219830.44731262437785635No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCGC218450.4445045844304359No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA212880.4331706840629489No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCG199590.4061280384823561No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA197950.4027909475303492No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA195900.3986195838403405No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA178830.363885350577683No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT178650.3635190844975847No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC172970.3519613548589265No Hit
CCACTACGCCTTGGCCGTACAGCAGTCTGCCGCAC165930.3376362815039699No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA157370.32021829458373857No Hit
TTCAAGTAATCCAGCTATCCGAGCGGAACCGGCAT156660.31877357837890635No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG144750.294538972745734No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT144100.29321634523426787No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA139220.28328646484049114No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC134910.27451642703369245No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA134300.2732751919844703No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC132710.27003984161026845No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA116240.236526495281272No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT111200.226271045038519No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA109420.2226490804686578No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA105530.21473366351542184No Hit
TCAGTGCACTACAGAACTTTGTCGCCTTGGCCGTA102050.20765251930018763No Hit
ACAGTAGTCTGCACATTGGTTCGCCTTGGCCGTAC100220.20392881415252131No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA99020.2014870402851992No Hit
AACATTCAACGCTGTCGGTGAGTCGCCTTGGCCGT97990.19939118438241435No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTCGC94940.19318500913630388No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG94120.19151646366030042No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT85770.17452578716685047No Hit
GCCGCCGGTGAAATACCACTACTCTGACGCCTTGG84020.17096486694367236No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACCGC80280.16335467172385165No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT77690.15808450979354802No Hit
TGAGATGAAGCACTGTAGCTCACGCCTTGGCCGTA74430.1514510241206562No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA74170.1509219731160697No Hit
CAGCAGCAATTCATGTTTTGACGCCTTGGCCGTAC73630.14982317487577473No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA73120.14878542098216285No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT71930.14636399523040172No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA68250.13887588870394713No Hit
GCCGCCGGTGAAATACCACTACCGCCTTGGCCGTA67750.13785848292589623No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA67670.1376956980014081No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC65340.1329545870756909No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC64850.13195752941320105No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC63030.1282541723810958No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA62040.12623970894055503No Hit
AGAGGTAGTAGGTTGCATAGTTCGCCTTGGCCGTA61830.12581239851377365No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA61610.12536473997143124No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA61090.12430663796225831No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT61070.1242659417311363No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG60810.12373689072654982No Hit
CGCCTTGGCCGTACAGCAGTCTGCCGCACAGACAC59200.12046084412122593No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA57920.11785628532941564No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCG56350.11466163118633584No Hit
TGTAAACATCCTACACTCAGCTCGCCTTGGCCGTA55640.11321691498150355No Hit
CATGCCTTGAGTGTAGGACCGTCGCCTTGGCCGTA55300.11252507905242896No Hit
TCCCTGAGACCCTACAGGTGTCATAAGGTTAATGC55230.11238264224350182No Hit
AAACCGTTACCATTACTGAGTCGCCTTGGCCGTAC54890.11169080631442721No Hit
ACAGTAGTCTGCACATTGGTTACGCCTTGGCCGTA54470.11083618546086446No Hit
TGTCAGTTTGTCAAATACCCCATCGCCTTGGCCGT54140.11016469764735087No Hit
TCAGTGCACTACAGAACTTTGTCCGCCTTGGCCGT53660.10918798810042202No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT53300.10845545594022539No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA53240.10833336724685927No Hit
TGAGGTAGTAGGTTGTATAGTTCGCAGGTTAATGC51680.10515906121934047No Hit
TGTAAACATCCTACACTCTCAGCTCGCCTTGGCCG51370.10452826963694892No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT51240.10426374413465568No Hit
TGTGCAAATCTATGCAAAACTGACGCCTTGGCCGT50990.10375504124563023No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC50310.10237136938748104No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCGC49710.10115048245381995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)