FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002406258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906264
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC1555515.352266690156159No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT899913.0964495998987016No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA771502.654610868111087No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA507551.7464001893840337No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT457801.5752182183036367No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA402391.3845610722219317No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA375841.2932066735850563No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT358901.234918782326726No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN339281.167409430113713No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA290210.9985672327083843No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN280590.9654663168934412No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT273830.942206213888346No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA243970.8394626228037095No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA242010.8327185692696878No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC231230.7956262748325684No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG213030.7330029205880815No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT197050.6780182392239659No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC170680.5872831924422558No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA154750.5324705532601305No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA151320.5206684595755926No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT147310.5068706765799667No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135780.4671977494129921No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA135150.46503001791991366No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA134450.4626214273720488No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN129340.4450387163726351No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA122170.42036786747521904No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT113850.391740048392025No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT107340.3693401562968815No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA103400.355783232356042No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC97120.33417473429805417No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA81080.27898360231555014No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT80430.2767470539496756No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA77140.2654266783747106No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC75840.26095358164296156No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT75550.25995573698741753No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT75330.25919875138666No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC73820.25400307749055145No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA66910.2302268479394852No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG65460.22523762466176508No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC62920.21649788181665533No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT62720.21580971308869393No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT61910.21302262974045028No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA61530.2117151091573236No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC60140.20693233649799192No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA60000.20645061838841894No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC58120.1999818323455818No Hit
TCAGTGCACTACAGAACTTTGCGCCTTGGCCGTAC55100.18959048455336475No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA51610.17758194025043836No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA51050.17565506781214646No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA50490.17372819537385453No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA48590.16719059245822127No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC47280.16268308729007414No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA45360.15607666750164473No Hit
TCTCACACAGAAATCGCACCCGCGCCTTGGCCGTA44980.15476914691851806No Hit
TGAGGTAGTAGGTTGTATAGTTCGCCTTGGCCGTA44970.15473473848212No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA43170.14854121993046743No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG43090.14826595243928287No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG41010.14110899766848436No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA40620.13976706864895963No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA40600.1396982517761635No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA39800.1369455768643179No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT39510.13594773220877387No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT39170.13477784537123952No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA37540.12916927023835412No Hit
TAGCAGCACAGAAATATTGGCACGCCTTGGCCGTA37350.12851550994679078No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT36680.12621014470812011No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC36010.12390477946944943No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC35590.1224596251407305No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC34850.11991340084727332No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATCA33890.11661019095305863No Hit
CGCCTTGGCCGTACAGCAGTCTTCGCTACAGATGT33880.11657578251666058No Hit
TGAGGTAGTAGGTTGTATAGTCGCCTTGGCCGTAC33650.11578438847950495No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC32690.11248117858529026No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC31920.10983172898263888No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC31910.1097973205462408No Hit
TCTCCCAACCCTTGTACCAGTGTCGCCTTGGCCGT31880.1096940952370466No Hit
GCTAAACCTAGCCCCAAACCCCGCCTTGGCCGTAC31390.10800808185354117No Hit
GTCCAGTTTTCCCAGGAATCCCACGCCTTGGCCGT31130.10711346250719135No Hit
AACCCGTAGATCCGATCTTGTGACGCCTTGGCCGT31120.1070790540707933No Hit
TGGCTCAGTTCAGCAGGAACAGACGCCTTGGCCGT29990.1031909007578114No Hit
GCTAAACCTAGCCCCAAACCCACGCCTTGGCCGTA29990.1031909007578114No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC29490.10147047893790792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)