FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4008046
Sequences flagged as poor quality0
Sequence length35
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT1091192.722498693877266No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA678011.6916223017400498No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC620611.5484103725356446No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN592391.4780019989790536No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA556741.389055914028931No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT506771.2643816962180574No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT470921.1749366149989295No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA451521.1265339769054548No Hit
GCCGCCGGTGAAATACCACTACTCGCCTTGGCCGT435591.0867889240792148No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT338200.8438026908872803No Hit
TATTGCACTTGTCCCGGCCTGTCGCCTTGGCCGTA333020.8308786875200533No Hit
GGCCGCCGGTGAAATACCACTACTCGCCTTGGCCG290250.7241683353933562No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC284720.7103710885553709No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA269820.6731958665145061No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC259570.6476223077280051No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA225840.5634665869603293No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCCA223820.5584267246433798No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA220100.5491453940398887No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN217220.5419598477662183No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN212090.5291605934662427No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAAAC208250.5195798651013487No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN193190.4820054460452799No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA181510.4528640639353939No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA171340.4274901036564949No Hit
TAGCTTATCAGACTGATGTTCTGTATTCCAATTTG127580.3183097199982236No Hit
TGTGCAAATCCATGCAAAACTGACGCCTTGGCCGT127180.3173117274602138No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA126390.31534069219764443No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCTAC125190.31234671458361507No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA121590.3033647817415269No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA120800.30139374647895756No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAAAC118670.2960794362140554No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA117920.2942082002052871No Hit
ATCACATTGCCAGGGATTTCCCGCCTTGGCCGTAC117560.2933100069210783No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA115490.28814539553687757No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGGTGT114980.2868729550509151No Hit
GTCCAGTTTTCCCAGGAATCGCCTTGGCCGTACAG104440.26057585167435704No Hit
TAGCTTATCAGACTGATGTTCTGGCGGAACCGGGT103920.2592784613749443No Hit
TAGCTTATCAGACTGATGTTGACATAAGTGGCCCA103640.2585798665983375No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA96050.2396429581896016No Hit
ATCACATTGCCAGGGATTTCCGCCTTGGCCGTACA95060.2371729266580274No Hit
ATCACATTGCCAGGGATTACCACTCGCCTTGGCCG92630.2311101219896179No Hit
TTCAAGTAATCCAGGATAGGCTCGCCTTGGCCGTA91000.22704330239722795No Hit
GTCCAGTTTTCCCAGGAATCCCTTCGCCTTGGCCG81160.20249268596218706No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC76940.19196386468618376No Hit
CGCGACCTCAGATCAGACGTCGCCTTGGCCGTACA72720.1814350434101804No Hit
TAGCTTATCAGACTGATGTTCTGTATTCACCGGGT67100.16741324825114282No Hit
AAACCGTTACCATTACTGAGTTCGCCTTGGCCGTA66220.16521766466752127No Hit
TAGCTTATCAGACTGATGTTCTGGCGGACAATTTG65390.16314683015115097No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT64270.16035245104472354No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCAC63280.1578824195131493No Hit
TCCCTGAGACCCTTTAACCTGTGACGCCTTGGCCG62930.15700917604239073No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA62450.155811584996779No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA62180.15513794003362236No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT59220.1477527952523499No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC56890.14193948871844286No Hit
TAGCTTATCAGACTGATGAACTGTATTCCAATTTG55060.13737367285704805No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC54540.13607628255763532No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGCGGCATG53760.13413019710851623No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT52880.13193461352489466No Hit
ATCACATTGCCAGGGATTTCCAACGCCTTGGCCGT52620.1312859183751883No Hit
GTCCAGTTTTCCCAGGAATCCGCCTTGGCCGTACA50790.1267201025137935No Hit
TAGCTTATCAGACTGATGTTGACCGTTCCAATTTG49960.12464926799742318No Hit
TAGCTTATCAGACTGATGTTGACCGCCTGTTAACA48090.11998365288222741No Hit
TAGCTTATCAGACTGATGTTGACATAAGGTTAGGT47350.11813736668690929No Hit
GGGCCGCCGGTGAAATACCACTACTCGCCTTGGCC47240.11786291873895659No Hit
TAGCACCATTTGAAATCAGTGTCGCCTTGGCCGTA47080.11746372172375266No Hit
TAGCTTATCAGACTGATGAAGACATAAGGTTAAAC46430.11584198384948677No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA45950.114644392803875No Hit
CGCCTTGGCCGTACAGCAGCTTACACCACAGGACA45800.11427014560212133No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA44390.11075222190563683No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC44070.10995382787522899No Hit
TAGCTTATCAGACTGATGTTGACATGGAACCGGGT42860.10693490044774935No Hit
TAGCTTATCAGACTGATGTTGACACGCCTTGGCCG42320.10558761052143614No Hit
CCCCCACAACCGCGCTTGACTAGCCGCCTTGGCCG41140.10264353253430723No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT40580.10124634298109353No Hit
TGGCTCAGTTCAGCAGGAACAGAGCGGAACCGGCA40430.10087209577933985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)