FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002406260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4647443
Sequences flagged as poor quality0
Sequence length35
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC1566583.3708428484222397No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT1205132.593103347367574No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT809401.741602855591774No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC663631.427946507358993No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA658741.417424592404899No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN640071.3772519641445844No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA547431.1779165446461635No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN513621.105166862724298No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA395800.8516511122352657No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT374860.8065940776465682No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT330630.7114234644728294No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA324960.6992232072561192No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA290510.6250964239905686No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAAATGC284100.6113038933452223No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA276440.594821711637991No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT202450.4356158859828942No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC200490.43139851311785854No Hit
CTGACCTATGAATTGACAGCTGTATTCCAAGGCAT197750.4255027979902066No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGGGCAT195830.42137149395915124No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT192420.414034125862329No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN189080.4068473782249723No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172210.3705478474937724No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC161340.3471586418596205No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG160940.34629795351981724No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC160490.34532967913753865No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAGCATG145420.3129032459354531No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA140950.30328505373815234No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC133500.2872547334093178No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA133220.2866522515714555No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT131140.2821766722044789No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA130670.28116536340521014No Hit
CTGACCTATGAATTGACAGCTTATAGGTTAATGCA127540.27443047714625013No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA118300.2545485764967962No Hit
CTGACCTATGAATTGACAGCTATTCCAATTTGCAC116840.25140706405651453No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAT115140.2477491386123509No Hit
TGGCAGTGTCTTAGCTGGTTGTCGCCTTGGCCGTA114830.24708210514900342No Hit
TGTCAGTTTGTCAAATACCCCAACGCCTTGGCCGT110030.23675384507136507No Hit
CTGACCTATGAATTGACAGCTGTATTCCATTTACG108290.23300985079322112No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA108070.23253647220632936No Hit
CTGACCTATGAATTGACAGCTGTATTCCATCCGTA101810.2190666996884093No Hit
TGACCTATGAATTGACAGCCACGCCTTGGGGCATG101420.21822752855710117No Hit
GTTTCCGTAGTGTAGTGGTTATCCGCCTTGGCCGT97780.21039526466489206No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC96830.20835112985785947No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA96750.20817899218989883No Hit
CTGACCTATGAATTGACAGCTTATGAACCGATGCA95070.2045641011627254No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG93980.20221872543676167No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA93820.20187445010084037No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA90080.19382701412368047No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA84920.1827241345402192No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC84280.1813470331965341No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC84120.18100275786061282No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT83560.17979779418488834No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG82860.17829158959023272No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA79640.17136304845481698No Hit
AGCAGCATTGTACAGGGCTATGACGCAGGTATTAC75660.16279919947377514No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC75100.16159423579805068No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT74450.16019561724587048No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA73880.15896913636165091No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA73820.15884003311068043No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC71830.1545581086201595No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC71400.15363286865487108No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA69930.1504698390060943No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGAATGC69400.14932942695585508No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGC68830.14810294607163552No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCCTA68630.14767260190173392No Hit
CTGACCTATGAATTGACAGCTATTCCAATTCATGT68260.14687646518741598No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA67370.14496143363135383No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC66450.1429818504498065No Hit
TCTCCCAACCCTTGTACCAGTGACGCCTTGGCCGT65730.14143261143816074No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC65510.14095923285126896No Hit
TGACCTATGAATTGACAGCCACGCCTTGGGGCATT65010.13988337242651497No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC64810.13945302825661338No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCTCG63510.136655791152253No Hit
CTGACCTATGAATTGACAGCCACGCCTTGCCCGTA61360.13202959132581077No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA61340.1319865569088206No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC60500.1301791113952339No Hit
CGCCTTGGCCGTACAGCAGTCATCCGCCTAGCGAT57240.12316450142583783No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA54290.11681692491978923No Hit
CTGACCTATGAATTGACAGCTGTATTCCATAATGC53710.11556892682707458No Hit
TGACCTATGAATTGACAGCCAGCGCAGGTTAATGC52180.11227679392732735No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC50140.10788728339433103No Hit
CTGACCTATGAATTGACAGCTGTATCTTGGGGCAT48030.10334715240186915No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC47920.10311046310842327No Hit
CTGACCTATGAATTGACAGCTGTATCTTGCTTACG47750.10274467056400692No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC47710.1026586017300266No Hit
TACCCTGTAGAACCGAATTTGTCGCCTTGGCCGTA47300.10177639618172832No Hit
TAGCACCATTTGAAATCGGTCGCCTTGGCCGTACA47210.10158274130527259No Hit
TGACCTATGAATTGACAGCCACGCCTGTTTTACGG46700.10048536367202351No Hit
TGAGGTAGTAGTTTGTACAGTCGCCTTGGCCGTAC46500.10005501950212192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)