FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002406262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002406262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3830920
Sequences flagged as poor quality0
Sequence length35
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTATGAATTGACAGCCCGCCTTGGCCGTAC46961412.258517536257608No Hit
CTGACCTATGAATTGACAGCCACGCCTTGGCCGTA1606524.193561859814353No Hit
CTGACCTATGAATTGACAGCCGCCTTGGCCGTACA819272.1385724577908176No Hit
TGACCTATGAATTGACAGCCACGCCTTGGCCGTAC772612.016774038612135No Hit
TGACCTATGAATTGACAGCCAGCGCCTTGGCCGTA736621.9228279368924437No Hit
CAACGGAATCCCAAAAGCAGCTCGCCTTGGCCGTA662941.7304981571006444No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN541441.4133419648543952No Hit
ATGACCTATGAATTGACAGACACGCCTTGGCCGTA451151.1776544537604545No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN424011.106809852463638No Hit
GTCCAGTTTTCCCAGGAATCCCTCGCCTTGGCCGT415391.0843087300178547No Hit
TCCCTGAGACCCTTTAACCTGTCGCCTTGGCCGTA383080.99996867593163No Hit
ATGACCTATGAATTGACAGACCGCCTTGGCCGTAC347560.9072494335564303No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACA323710.8449928476710555No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGGCCGT299620.7821097804182808No Hit
CACGCTCATGCACACACCCACACGCCTTGGCCGTA296030.7727386632975891No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGCCGTA289610.7559802867196391No Hit
TGGCTCAGTTCAGCAGGAACAGTCGCCTTGGCCGT274450.7164075470122059No Hit
CTGACCTATGAATTGACAGCTTATTCCAATTACGT251330.6560565086193394No Hit
TCTCCCAACCCTTGTACCAGTGCGCCTTGGCCGTA233430.6093314399674229No Hit
TGACCTATGAATTGACAGCCAGACGCCTTGGCCGT232830.6077652365489229No Hit
ATGACCTATGAATTGACAGACGCCTTGGCCGTACA205160.5355371555657649No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN186900.4878723648627484No Hit
CTGACCTATGAATTGACAGCCCGCCTTGGCTACGT178700.46646758480991507No Hit
AGCAGCATTGTACAGGGCTATGACGCCTTGCGGCA155770.406612510832907No Hit
TAGCACCATCTGAAATCGGTTACGCCTTGGCCGTA141330.3689192152276738No Hit
CTGACCTATGAATTGACAGCCTCGCCTTGGCCGTA137360.35855616927526546No Hit
TGACCTATGAATTGACAGCCAGTCGCCTTGGCCGT132600.3461309554884988No Hit
TCCCCGGCATCTCCACCACGCCTTGGCCGTACAGC129790.3387959028118572No Hit
GTCCAGTTTTCCCAGGAATCCCGCCTTGGCCGTAC109730.2864325018533407No Hit
TAGCACCATCTGAAATCGGTTCGCCTTGGCCGTAC109050.284657471312374No Hit
TCCCTGAGACCCTAACTTGTGACGCCTTGGCCGTA104420.27257160159961574No Hit
TGTCAGTTTGTCAAATACCCCACGCCTTGGCCGTA101950.26612406419345747No Hit
ATCACATTGCCAGGGATTTCCACGCCTTGGCCGTA101370.26461006755557415No Hit
CTGACCTATGAATTGACAGCCACTAAGGTTAATGC101010.2636703455044741No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98490.2570922911467741No Hit
CTGACCTATGAATTGACAGCTGTATTCCAATTACG88990.23229407035385755No Hit
TCCCCGGCACCTCCACCACGCCTTGGCCGTACAGC87670.22884842283315757No Hit
TAGCACCATTTGAAATCAGTGTTCGCCTTGGCCGT85530.22326229730717426No Hit
ATCACATTGCCAGGGATTACCACGCCTTGGCCGTA85290.22263581593977427No Hit
CAGTGCAATGATGAAAGGGCATCGCCTTGGCCGTA80540.21023670554331597No Hit
TGACCTATGAATTGACAGCCCGCCTTGGCCGTACC76700.20021300366491598No Hit
CTGACCTATGAATTGACAGCGCCTTGGCCGTACAG73860.19279964081734938No Hit
TAGCACCATTTGAAATCAGTCGCCTTGGCCGTACA72200.18846647802616606No Hit
CTGACCTATGAATTGACAGCTTATTCCAATCGTAC72110.18823154751339105No Hit
CAACGGAATCCCAAAAGCAGCCGCCTTGGCCGTAC71840.18752675597506605No Hit
CATTATTACTTTTGGTACGCGCGCCTTGGCCGTAC71650.1870307915592077No Hit
CTGACCTATGAATTGACAGCTATTCCAATTACGTG67410.17596295406847443No Hit
CTGACCTATGAATTGACAGCCCGAAGGTTAATGCA66590.17382247606319107No Hit
TGACCTATGAATTGACAGCCGCCTTGGCCGTACAG65100.16993307090724943No Hit
TAGCAGCACATCATGGTTTACACGCCTTGGCCGTA64470.16828855731782444No Hit
TGAGGTAGTAGTTTGTACAGTTCGCAGGTTAATGC62960.16434694538126612No Hit
TCAGGCTCAGTCCCCTCCCGATCGCCTTGGCCGTA62390.16285905213369112No Hit
GTCCAGTTTTCCCAGGAATCCCCGCCTTGGGGCAT61700.16105791820241613No Hit
TAGCTTATCAGACTGATGTTGACCGCCTTGGCCGT58210.15194783498480782No Hit
TGAGGTAGTAGATTGTATAGTTCGCCTTGGCCGTA57780.15082538920154948No Hit
ACCACAGGGTAGAACCACGGACGCCTTGGCCGTAC57060.1489459450993495No Hit
TGACCTATGAATTGACAGCCCGCCTTGCGGCATGT56880.1484760840737995No Hit
TGAGGTAGTAGATTGTATAGTCGCCTTGGCCGTAC56430.1473014315099245No Hit
TGAGGTAGTAGTTTGTACAGTTCGCCTTGGCCGTA56130.1465183298006745No Hit
ATCCCACCGCTGCCACCACGCCTTGGCCGTACAGC55580.14508264333371618No Hit
AGCAGCATTGTACAGGGCTATGTCGCCTTGGCCGT54030.14103661783592453No Hit
TGGCTCAGTTCAGCAGGAACAGCGCCTTGGCCGTA53490.13962703475927454No Hit
TAGCACCATCTGAAATCGGTCGCCTTGGCCGTACA52310.1365468347028912No Hit
ACCACAGGGTAGAACCACGGACCGCCTTGGCCGTA50630.13216146513109123No Hit
CTGACCTATGAATTGACAGCCCGCGAACCGGCATG50520.13187432783769956No Hit
TAGCACCATTTGAAATCGGTTACGCCTTGGCCGTA50040.13062136510289957No Hit
TCTCCCAACCCTTGTACCAGTCGCCTTGGCCGTAC49750.12986436678395788No Hit
CATTATTACTTTTGGTACGCGACGCCTTGGCCGTA48730.1272018209725079No Hit
ATCACATTGCCAGGGATTACCCGCCTTGGCCGTAC48540.12670585655664957No Hit
TAGCACCATTTGAAATCGGTTCGCCTTGGCCGTAC47700.12451317177074958No Hit
CCCAGTGTTCAGACTACCTGTCGCCTTGGCCGTAC47170.12312969208440791No Hit
CACGCTCATGCACACACCCACGCCTTGGCCGTACA46890.12239879715577459No Hit
TGAGGTAGTAGATTGTATAGTTCGCAGGTTAATGC45720.1193447004896996No Hit
TACCCTGTAGATCCGAATTTGTCGCCTTGGCCGTA44740.11678656823948294No Hit
ACCCCACTCCTGGTACCACGCCTTGGCCGTACAGC43020.11229678510644964No Hit
TCCCTGAGACCCTTTAACCTGCGCCTTGGCCGTAC42260.11031292744301631No Hit
TCTCACACAGAAATCGCACCCGTCGCCTTGGCCGT42020.1096864460756163No Hit
GGTTCCATAGTGTAGTGGTTATCCGCCTTGGCCGT39770.10381318325624132No Hit
TGGCTCAGTTCAGCAGGAACGCCTTGGCCGTACAG38770.101202844225408No Hit
CTGACCTATGAATTGACAGCCATCGCCTTGGCCGT38360.10013260522276632No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)